Abstract
We measured the anisotropy of Tb 3+ and Eu 3+ in various chelate complexes bound with or without a carbostyril antenna. The anisotropy of the lanthanides depends on the chelate as well as whether the antenna is present. The anisotropy of Eu 3+ bound to only a chelate is high while for Tb 3+ the anisotropy is nearly zero. For Eu 3+, the anisotropy decreases upon addition of the antenna, while for Tb 3+ the anisotropy slightly increases once the antenna is attached. A theoretical model is discussed to account for the measured anisotropy of the Eu 3+ bound to the chelate and how the addition of the antenna affects the anisotropy. While the transitions within Tb 3+ are more complicated than those in Eu 3+, the theoretical model is also extended to Tb 3+ to explain qualitatively how the addition of the antenna affects the measured anisotropy of the terbium complex. Because of their low anisotropy, both Tb 3+ and Eu 3+, when bound to a chelate with an antenna, are ideal luminescent probes in resonance energy transfer experiments for measuring nanometer scale distances. Our results indicate that it may be possible to construct a probe with Eu 3+ that retains its high anisotropy once the antenna is added to the chelate complex. This probe could measure rotational motion on the millisecond lifetime.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 12862-12873 |
| Number of pages | 12 |
| Journal | Journal of Physical Chemistry B |
| Volume | 107 |
| Issue number | 46 |
| State | Published - Nov 20 2003 |
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ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Surfaces, Coatings and Films
- Materials Chemistry
Cite this
Emission Polarization of Europium and Terbium Chelates. / Reifenberger, Jeff G.; Snyder, Gregory E.; Baym, Gordon; Selvin, Paul R.
In: Journal of Physical Chemistry B, Vol. 107, No. 46, 20.11.2003, p. 12862-12873.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Emission Polarization of Europium and Terbium Chelates
AU - Reifenberger, Jeff G.
AU - Snyder, Gregory E.
AU - Baym, Gordon
AU - Selvin, Paul R.
PY - 2003/11/20
Y1 - 2003/11/20
N2 - We measured the anisotropy of Tb 3+ and Eu 3+ in various chelate complexes bound with or without a carbostyril antenna. The anisotropy of the lanthanides depends on the chelate as well as whether the antenna is present. The anisotropy of Eu 3+ bound to only a chelate is high while for Tb 3+ the anisotropy is nearly zero. For Eu 3+, the anisotropy decreases upon addition of the antenna, while for Tb 3+ the anisotropy slightly increases once the antenna is attached. A theoretical model is discussed to account for the measured anisotropy of the Eu 3+ bound to the chelate and how the addition of the antenna affects the anisotropy. While the transitions within Tb 3+ are more complicated than those in Eu 3+, the theoretical model is also extended to Tb 3+ to explain qualitatively how the addition of the antenna affects the measured anisotropy of the terbium complex. Because of their low anisotropy, both Tb 3+ and Eu 3+, when bound to a chelate with an antenna, are ideal luminescent probes in resonance energy transfer experiments for measuring nanometer scale distances. Our results indicate that it may be possible to construct a probe with Eu 3+ that retains its high anisotropy once the antenna is added to the chelate complex. This probe could measure rotational motion on the millisecond lifetime.
AB - We measured the anisotropy of Tb 3+ and Eu 3+ in various chelate complexes bound with or without a carbostyril antenna. The anisotropy of the lanthanides depends on the chelate as well as whether the antenna is present. The anisotropy of Eu 3+ bound to only a chelate is high while for Tb 3+ the anisotropy is nearly zero. For Eu 3+, the anisotropy decreases upon addition of the antenna, while for Tb 3+ the anisotropy slightly increases once the antenna is attached. A theoretical model is discussed to account for the measured anisotropy of the Eu 3+ bound to the chelate and how the addition of the antenna affects the anisotropy. While the transitions within Tb 3+ are more complicated than those in Eu 3+, the theoretical model is also extended to Tb 3+ to explain qualitatively how the addition of the antenna affects the measured anisotropy of the terbium complex. Because of their low anisotropy, both Tb 3+ and Eu 3+, when bound to a chelate with an antenna, are ideal luminescent probes in resonance energy transfer experiments for measuring nanometer scale distances. Our results indicate that it may be possible to construct a probe with Eu 3+ that retains its high anisotropy once the antenna is added to the chelate complex. This probe could measure rotational motion on the millisecond lifetime.
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M3 - Article
AN - SCOPUS:0344531011
VL - 107
SP - 12862
EP - 12873
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
SN - 1520-6106
IS - 46
ER -