Emerging technologies to create inducible and genetically defined porcine cancer models

Lawrence B Schook, Laurie Rund, Karine R. Begnini, Mariana H. Remião, Fabiana K. Seixas, Tiago Collares

Research output: Contribution to journalShort survey

Abstract

There is an emerging need for new animal models that address unmet translational cancer research requirements. Transgenic porcine models provide an exceptional opportunity due to their genetic, anatomic, and physiological similarities with humans. Due to recent advances in the sequencing of domestic animal genomes and the development of new organism cloning technologies, it is now very feasible to utilize pigs as a malleable species, with similar anatomic and physiological features with humans, in which to develop cancer models. In this review, we discuss genetic modification technologies successfully used to produce porcine biomedical models, in particular the Cre-loxP System as well as major advances and perspectives the CRISPR/Cas9 System. Recent advancements in porcine tumor modeling and genome editing will bring porcine models to the forefront of translational cancer research.

Original languageEnglish (US)
Article number28
JournalFrontiers in Genetics
Volume7
Issue numberFEB
DOIs
StatePublished - Jan 1 2016

Fingerprint

Swine
Technology
Translational Medical Research
Neoplasms
Clustered Regularly Interspaced Short Palindromic Repeats
Domestic Animals
Organism Cloning
Animal Models
Genome

Keywords

  • Biotechnology
  • Cancer
  • Genome editing
  • Oncopigs
  • Transgenesis

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Genetics(clinical)

Cite this

Emerging technologies to create inducible and genetically defined porcine cancer models. / Schook, Lawrence B; Rund, Laurie; Begnini, Karine R.; Remião, Mariana H.; Seixas, Fabiana K.; Collares, Tiago.

In: Frontiers in Genetics, Vol. 7, No. FEB, 28, 01.01.2016.

Research output: Contribution to journalShort survey

Schook, Lawrence B ; Rund, Laurie ; Begnini, Karine R. ; Remião, Mariana H. ; Seixas, Fabiana K. ; Collares, Tiago. / Emerging technologies to create inducible and genetically defined porcine cancer models. In: Frontiers in Genetics. 2016 ; Vol. 7, No. FEB.
@article{723f14dad49c47b2af80c8f2a4919cd1,
title = "Emerging technologies to create inducible and genetically defined porcine cancer models",
abstract = "There is an emerging need for new animal models that address unmet translational cancer research requirements. Transgenic porcine models provide an exceptional opportunity due to their genetic, anatomic, and physiological similarities with humans. Due to recent advances in the sequencing of domestic animal genomes and the development of new organism cloning technologies, it is now very feasible to utilize pigs as a malleable species, with similar anatomic and physiological features with humans, in which to develop cancer models. In this review, we discuss genetic modification technologies successfully used to produce porcine biomedical models, in particular the Cre-loxP System as well as major advances and perspectives the CRISPR/Cas9 System. Recent advancements in porcine tumor modeling and genome editing will bring porcine models to the forefront of translational cancer research.",
keywords = "Biotechnology, Cancer, Genome editing, Oncopigs, Transgenesis",
author = "Schook, {Lawrence B} and Laurie Rund and Begnini, {Karine R.} and Remi{\~a}o, {Mariana H.} and Seixas, {Fabiana K.} and Tiago Collares",
year = "2016",
month = "1",
day = "1",
doi = "10.3389/fgene.2016.00028",
language = "English (US)",
volume = "7",
journal = "Frontiers in Genetics",
issn = "1664-8021",
publisher = "Frontiers Media S. A.",
number = "FEB",

}

TY - JOUR

T1 - Emerging technologies to create inducible and genetically defined porcine cancer models

AU - Schook, Lawrence B

AU - Rund, Laurie

AU - Begnini, Karine R.

AU - Remião, Mariana H.

AU - Seixas, Fabiana K.

AU - Collares, Tiago

PY - 2016/1/1

Y1 - 2016/1/1

N2 - There is an emerging need for new animal models that address unmet translational cancer research requirements. Transgenic porcine models provide an exceptional opportunity due to their genetic, anatomic, and physiological similarities with humans. Due to recent advances in the sequencing of domestic animal genomes and the development of new organism cloning technologies, it is now very feasible to utilize pigs as a malleable species, with similar anatomic and physiological features with humans, in which to develop cancer models. In this review, we discuss genetic modification technologies successfully used to produce porcine biomedical models, in particular the Cre-loxP System as well as major advances and perspectives the CRISPR/Cas9 System. Recent advancements in porcine tumor modeling and genome editing will bring porcine models to the forefront of translational cancer research.

AB - There is an emerging need for new animal models that address unmet translational cancer research requirements. Transgenic porcine models provide an exceptional opportunity due to their genetic, anatomic, and physiological similarities with humans. Due to recent advances in the sequencing of domestic animal genomes and the development of new organism cloning technologies, it is now very feasible to utilize pigs as a malleable species, with similar anatomic and physiological features with humans, in which to develop cancer models. In this review, we discuss genetic modification technologies successfully used to produce porcine biomedical models, in particular the Cre-loxP System as well as major advances and perspectives the CRISPR/Cas9 System. Recent advancements in porcine tumor modeling and genome editing will bring porcine models to the forefront of translational cancer research.

KW - Biotechnology

KW - Cancer

KW - Genome editing

KW - Oncopigs

KW - Transgenesis

UR - http://www.scopus.com/inward/record.url?scp=84962623805&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962623805&partnerID=8YFLogxK

U2 - 10.3389/fgene.2016.00028

DO - 10.3389/fgene.2016.00028

M3 - Short survey

VL - 7

JO - Frontiers in Genetics

JF - Frontiers in Genetics

SN - 1664-8021

IS - FEB

M1 - 28

ER -