Embryonic expression of pre-initiation DNA replication factors in Xenopus laevis

Brian E. Walter, Jonathan Joseph Henry

Research output: Contribution to journalArticle

Abstract

We examined the expression of various DNA replication factors, including: cdc45, the factors of the GINS heterotetramer (Sld5, Psf1, Psf2, Psf3), and PCNA, in Xenopus laevis during embryonic development via whole mount in situ hybridization. For the most part, these factors were expressed in similar patterns, with some subtle variations, throughout development within the anterior CNS, pharyngeal arches, and various placodes. More significant variations were also observed, including expression of only Psf1 and Psf2 in the pronephros and unique Psf2 expression in the somitic mesoderm. Overall, these results suggest that common regulatory mechanisms are involved in the transcriptional deployment of these factors. Significantly, expression of these factors does not directly coincide with elevated patterns of DNA replication, suggesting that different replication factors are utilized in different developmental regions.

Original languageEnglish (US)
Pages (from-to)81-89
Number of pages9
JournalGene Expression Patterns
Volume5
Issue number1
DOIs
StatePublished - Nov 1 2004

Fingerprint

Xenopus laevis
DNA Replication
Pronephros
Branchial Region
Proliferating Cell Nuclear Antigen
Mesoderm
Embryonic Development
In Situ Hybridization

Keywords

  • Development
  • GINS
  • In situ hybridization
  • Lens
  • PCNA
  • Psf1
  • Psf2
  • Psf3
  • Sld5
  • Transdifferentiation
  • Xenopus
  • cdc45

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Developmental Biology

Cite this

Embryonic expression of pre-initiation DNA replication factors in Xenopus laevis. / Walter, Brian E.; Henry, Jonathan Joseph.

In: Gene Expression Patterns, Vol. 5, No. 1, 01.11.2004, p. 81-89.

Research output: Contribution to journalArticle

@article{8c9b9515f3934ed091d6ad74cb1364cd,
title = "Embryonic expression of pre-initiation DNA replication factors in Xenopus laevis",
abstract = "We examined the expression of various DNA replication factors, including: cdc45, the factors of the GINS heterotetramer (Sld5, Psf1, Psf2, Psf3), and PCNA, in Xenopus laevis during embryonic development via whole mount in situ hybridization. For the most part, these factors were expressed in similar patterns, with some subtle variations, throughout development within the anterior CNS, pharyngeal arches, and various placodes. More significant variations were also observed, including expression of only Psf1 and Psf2 in the pronephros and unique Psf2 expression in the somitic mesoderm. Overall, these results suggest that common regulatory mechanisms are involved in the transcriptional deployment of these factors. Significantly, expression of these factors does not directly coincide with elevated patterns of DNA replication, suggesting that different replication factors are utilized in different developmental regions.",
keywords = "Development, GINS, In situ hybridization, Lens, PCNA, Psf1, Psf2, Psf3, Sld5, Transdifferentiation, Xenopus, cdc45",
author = "Walter, {Brian E.} and Henry, {Jonathan Joseph}",
year = "2004",
month = "11",
day = "1",
doi = "10.1016/j.modgep.2004.06.005",
language = "English (US)",
volume = "5",
pages = "81--89",
journal = "Gene Expression Patterns",
issn = "1567-133X",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Embryonic expression of pre-initiation DNA replication factors in Xenopus laevis

AU - Walter, Brian E.

AU - Henry, Jonathan Joseph

PY - 2004/11/1

Y1 - 2004/11/1

N2 - We examined the expression of various DNA replication factors, including: cdc45, the factors of the GINS heterotetramer (Sld5, Psf1, Psf2, Psf3), and PCNA, in Xenopus laevis during embryonic development via whole mount in situ hybridization. For the most part, these factors were expressed in similar patterns, with some subtle variations, throughout development within the anterior CNS, pharyngeal arches, and various placodes. More significant variations were also observed, including expression of only Psf1 and Psf2 in the pronephros and unique Psf2 expression in the somitic mesoderm. Overall, these results suggest that common regulatory mechanisms are involved in the transcriptional deployment of these factors. Significantly, expression of these factors does not directly coincide with elevated patterns of DNA replication, suggesting that different replication factors are utilized in different developmental regions.

AB - We examined the expression of various DNA replication factors, including: cdc45, the factors of the GINS heterotetramer (Sld5, Psf1, Psf2, Psf3), and PCNA, in Xenopus laevis during embryonic development via whole mount in situ hybridization. For the most part, these factors were expressed in similar patterns, with some subtle variations, throughout development within the anterior CNS, pharyngeal arches, and various placodes. More significant variations were also observed, including expression of only Psf1 and Psf2 in the pronephros and unique Psf2 expression in the somitic mesoderm. Overall, these results suggest that common regulatory mechanisms are involved in the transcriptional deployment of these factors. Significantly, expression of these factors does not directly coincide with elevated patterns of DNA replication, suggesting that different replication factors are utilized in different developmental regions.

KW - Development

KW - GINS

KW - In situ hybridization

KW - Lens

KW - PCNA

KW - Psf1

KW - Psf2

KW - Psf3

KW - Sld5

KW - Transdifferentiation

KW - Xenopus

KW - cdc45

UR - http://www.scopus.com/inward/record.url?scp=8844266081&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=8844266081&partnerID=8YFLogxK

U2 - 10.1016/j.modgep.2004.06.005

DO - 10.1016/j.modgep.2004.06.005

M3 - Article

C2 - 15533822

AN - SCOPUS:8844266081

VL - 5

SP - 81

EP - 89

JO - Gene Expression Patterns

JF - Gene Expression Patterns

SN - 1567-133X

IS - 1

ER -