TY - JOUR
T1 - Elucidation of the fucose metabolism of probiotic Lactobacillus rhamnosus GG by metabolomic and flux balance analyses
AU - Cheong, Yu Eun
AU - Kim, Jungyeon
AU - Jin, Yong Su
AU - Kim, Kyoung Heon
N1 - Funding Information:
This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food , Agriculture, Forestry, and Fisheries, funded by the Ministry of Agriculture, Food and Rural Affairs ( 32136–05-1-SB010 ) and the Mid-career Researcher Program through the National Research Foundation of Korea ( 2020R1A2B5B02002631 ). Facility support by the Institute of Biomedical and Food Safety at the CJ Food Safety Hall, Korea University, is acknowledged.
Publisher Copyright:
© 2022 Elsevier B.V.
PY - 2022/12/10
Y1 - 2022/12/10
N2 - Lactobacillus rhamnosus GG (LGG) is one of the most widely used probiotics because of its health benefits and safety. Fucose is among the most abundant hexoses in the human intestine, and LGG consumes fucose to produce energy or proliferate. However, no study has elucidated the metabolism by which LGG metabolizes fucose to produce energy, biomass, and extracellular metabolites. We used metabolomics and flux balance analysis to elucidate these mechanisms and highlight how they might affect the host. We found three different metabolic flux modes by which LGG anaerobically metabolizes fucose to produce energy and biomass. These metabolic flux modes differ from homolactic or heterolactic fermentation and account for the production of lactic acid, 1,2-propanediol, acetic acid, formic acid, and carbon dioxide as a result of fucose metabolism in LGG. We also used gas chromatography/time-of-flight mass spectrometry to identify a variety of short-chain fatty acids and organic acids secreted during fucose metabolism by LGG. Our study is the first to elucidate the unique fucose metabolism of LGG in anaerobic condition.
AB - Lactobacillus rhamnosus GG (LGG) is one of the most widely used probiotics because of its health benefits and safety. Fucose is among the most abundant hexoses in the human intestine, and LGG consumes fucose to produce energy or proliferate. However, no study has elucidated the metabolism by which LGG metabolizes fucose to produce energy, biomass, and extracellular metabolites. We used metabolomics and flux balance analysis to elucidate these mechanisms and highlight how they might affect the host. We found three different metabolic flux modes by which LGG anaerobically metabolizes fucose to produce energy and biomass. These metabolic flux modes differ from homolactic or heterolactic fermentation and account for the production of lactic acid, 1,2-propanediol, acetic acid, formic acid, and carbon dioxide as a result of fucose metabolism in LGG. We also used gas chromatography/time-of-flight mass spectrometry to identify a variety of short-chain fatty acids and organic acids secreted during fucose metabolism by LGG. Our study is the first to elucidate the unique fucose metabolism of LGG in anaerobic condition.
KW - Flux balance analysis
KW - Fucose metabolism
KW - Lactobacillus rhamnosus GG
KW - Metabolomics
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U2 - 10.1016/j.jbiotec.2022.11.002
DO - 10.1016/j.jbiotec.2022.11.002
M3 - Article
C2 - 36336085
AN - SCOPUS:85141753109
SN - 0168-1656
VL - 360
SP - 110
EP - 116
JO - Journal of Biotechnology
JF - Journal of Biotechnology
ER -