TY - JOUR
T1 - Eleven biosynthetic genes explain the majority of natural variation in carotenoid levels in maize grain
AU - Diepenbrock, Christine H.
AU - Ilut, Daniel C.
AU - Magallanes-Lundback, Maria
AU - Kandianis, Catherine B.
AU - Lipka, Alexander E.
AU - Bradbury, Peter J.
AU - Holland, James B.
AU - Hamilton, John P.
AU - Wooldridge, Edmund
AU - Vaillancourt, Brieanne
AU - Góngora-Castillo, Elsa
AU - Wallace, Jason G.
AU - Cepela, Jason
AU - Mateos-Hernandez, Maria
AU - Owens, Brenda F.
AU - Tiede, Tyler
AU - Buckler, Edward S.
AU - Rocheford, Torbert
AU - Buell, C. Robin
AU - Gore, Michael A.
AU - DellaPenna, Dean
N1 - Publisher Copyright:
VC American Society of Plant Biologists 2020. All rights reserved.
PY - 2021/4
Y1 - 2021/4
N2 - Vitamin A deficiency remains prevalent in parts of Asia, Latin America, and sub-Saharan Africa where maize (Zea mays) is a food staple. Extensive natural variation exists for carotenoids in maize grain. Here, to understand its genetic basis, we conducted a joint linkage and genome-wide association study of the US maize nested association mapping panel. Eleven of the 44 detected quantitative trait loci (QTL) were resolved to individual genes. Six of these were correlated expression and effect QTL (ceeQTL), showing strong correlations between RNA-seq expression abundances and QTL allelic effect estimates across six stages of grain development. These six ceeQTL also had the largest percentage of phenotypic variance explained, and in major part comprised the three to five loci capturing the bulk of genetic variation for each trait. Most of these ceeQTL had strongly correlated QTL allelic effect estimates across multiple traits. These findings provide an in-depth genome-level understanding of the genetic and molecular control of carotenoids in plants. In addition, these findings provide a roadmap to accelerate breeding for provitamin A and other priority carotenoid traits in maize grain that should be readily extendable to other cereals.
AB - Vitamin A deficiency remains prevalent in parts of Asia, Latin America, and sub-Saharan Africa where maize (Zea mays) is a food staple. Extensive natural variation exists for carotenoids in maize grain. Here, to understand its genetic basis, we conducted a joint linkage and genome-wide association study of the US maize nested association mapping panel. Eleven of the 44 detected quantitative trait loci (QTL) were resolved to individual genes. Six of these were correlated expression and effect QTL (ceeQTL), showing strong correlations between RNA-seq expression abundances and QTL allelic effect estimates across six stages of grain development. These six ceeQTL also had the largest percentage of phenotypic variance explained, and in major part comprised the three to five loci capturing the bulk of genetic variation for each trait. Most of these ceeQTL had strongly correlated QTL allelic effect estimates across multiple traits. These findings provide an in-depth genome-level understanding of the genetic and molecular control of carotenoids in plants. In addition, these findings provide a roadmap to accelerate breeding for provitamin A and other priority carotenoid traits in maize grain that should be readily extendable to other cereals.
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U2 - 10.1093/plcell/koab032
DO - 10.1093/plcell/koab032
M3 - Article
C2 - 33681994
AN - SCOPUS:85107030695
SN - 1040-4651
VL - 33
SP - 882
EP - 900
JO - Plant Cell
JF - Plant Cell
IS - 4
ER -