Elevated levels of cathepsin B in human glioblastoma cell lines.

S. Konduri, S. S. Lakka, A. Tasiou, N. Yanamandra, C. S. Gondi, D. H. Dinh, W. C. Olivero, M. Gujrati, J. S. Rao

Research output: Contribution to journalArticle

Abstract

Degradation of the extracellular matrix is a prerequisite for the invasive phenotype in glioma cells. Several proteases released by invading tumor cells seem to participate in the focal degradation of extracellular matrix proteins. Using enzymatic assays, Western blotting, and Northern blotting techniques, we investigated whether cathepsin B level was associated with malignant grade in seven human glioma cell lines. Cathepsin B activity and protein content levels were higher in glioblastoma cell lines than in anaplastic astrocytoma or low-grade glioma cell lines. Cathepsin B transcripts were overexpressed in glioblastoma cell lines relative to their expression in anaplastic astrocytoma and low-grade glioma cell lines. Cathepsin B promoter activity and amount of SP-1 complexes were much higher in glioblastoma cell lines than in anaplastic astrocytoma or low-grade glioma cell lines. Finally, E-64, an inhibitor of cathepsin B, inhibited both cathepsin B enzymatic activity and the invasiveness of glioblastoma cell lines. These results strongly support a role for cathepsin B in glioblastoma cell lines and suggest that inhibition of cathepsin B activity may be proven useful in cancer therapy.

Original languageEnglish (US)
Pages (from-to)519-524
Number of pages6
JournalInternational journal of oncology
Volume19
Issue number3
StatePublished - Jan 1 2001

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Cathepsin B
Glioblastoma
Cell Line
Glioma
Astrocytoma
Extracellular Matrix Proteins
Enzyme Assays
Northern Blotting
Extracellular Matrix
Neoplasms
Peptide Hydrolases
Western Blotting
Phenotype

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Konduri, S., Lakka, S. S., Tasiou, A., Yanamandra, N., Gondi, C. S., Dinh, D. H., ... Rao, J. S. (2001). Elevated levels of cathepsin B in human glioblastoma cell lines. International journal of oncology, 19(3), 519-524.

Elevated levels of cathepsin B in human glioblastoma cell lines. / Konduri, S.; Lakka, S. S.; Tasiou, A.; Yanamandra, N.; Gondi, C. S.; Dinh, D. H.; Olivero, W. C.; Gujrati, M.; Rao, J. S.

In: International journal of oncology, Vol. 19, No. 3, 01.01.2001, p. 519-524.

Research output: Contribution to journalArticle

Konduri, S, Lakka, SS, Tasiou, A, Yanamandra, N, Gondi, CS, Dinh, DH, Olivero, WC, Gujrati, M & Rao, JS 2001, 'Elevated levels of cathepsin B in human glioblastoma cell lines.', International journal of oncology, vol. 19, no. 3, pp. 519-524.
Konduri S, Lakka SS, Tasiou A, Yanamandra N, Gondi CS, Dinh DH et al. Elevated levels of cathepsin B in human glioblastoma cell lines. International journal of oncology. 2001 Jan 1;19(3):519-524.
Konduri, S. ; Lakka, S. S. ; Tasiou, A. ; Yanamandra, N. ; Gondi, C. S. ; Dinh, D. H. ; Olivero, W. C. ; Gujrati, M. ; Rao, J. S. / Elevated levels of cathepsin B in human glioblastoma cell lines. In: International journal of oncology. 2001 ; Vol. 19, No. 3. pp. 519-524.
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