Electrostatic changes in Lycopersicon esculentum root plasma membrane resulting from salt stress

Charles G. Suhayda, John L. Giannini, Donald P. Briskin, Michael C. Shannon

Research output: Contribution to journalArticle

Abstract

Salinity-induced alterations in tomato (Lypersicon esculentum Mill. cv Heinz 1350) root plasma membrane properties were studied and characterized using a membrane vesicle system. Equivalent rates of MgATP-dependent H + -transport activity were measured by quinacrine fluorescence (ΔpH) in plasma membrane vesicles isolated from control or salt-stressed (75 millimolar salt) tomato roots. However, when bis-[3-phenyl-5-oxoisoxazol-4-yl] pentamethine was used to measure MgATP-dependent membrane potential (Δψ) formation, salt-stressed vesicles displayed a 50% greater initial quench rate and a 30% greater steady state quench than control vesicles. This differential probe response suggested a difference in surface properties between control and salt-stressed membranes. Fluorescence titration of vesicles with the surface potential probe, 8-anilino-1-napthalenesulphonic acid (ANS) provided dissociation constants (K d ) of 120 and 76 micromolar for dye binding to control and salt-stressed vesicles, respectively. Membrane surface potentials (ψ o ) of-26.0 and -13.7 millivolts were calculated for control and salt-stressed membrane vesicles from the measured K d values and the calculated intrinsic affinity constant, K i . The concentration of cations and anions at the surface of control and salt-stressed membranes was estimated using ψ o values and the Boltzmann equation. The observed difference in membrane surface electrostatic properties was consistent with the measured differences in K + -stimulated kinetics of ATPase activity between control and salt-stressed vesicles and by the differential ability of Cl - ions to stimulate H + -transport activity. Salinity-induced changes in plasma membrane electrostatic properties may influence ion transport across the plasma membrane.

Original languageEnglish (US)
Pages (from-to)471-478
Number of pages8
JournalPlant physiology
Volume93
Issue number2
DOIs
StatePublished - Jun 1990

Fingerprint

Lycopersicon esculentum
Static Electricity
Solanum lycopersicum var. lycopersicum
salt stress
plasma membrane
Salts
Cell Membrane
salts
Membranes
Surface Properties
Salinity
Membrane Potentials
Adenosine Triphosphate
Fluorescence
tomatoes
fluorescence
quinacrine
dye binding
salinity
Quinacrine

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Electrostatic changes in Lycopersicon esculentum root plasma membrane resulting from salt stress. / Suhayda, Charles G.; Giannini, John L.; Briskin, Donald P.; Shannon, Michael C.

In: Plant physiology, Vol. 93, No. 2, 06.1990, p. 471-478.

Research output: Contribution to journalArticle

Suhayda, Charles G. ; Giannini, John L. ; Briskin, Donald P. ; Shannon, Michael C. / Electrostatic changes in Lycopersicon esculentum root plasma membrane resulting from salt stress. In: Plant physiology. 1990 ; Vol. 93, No. 2. pp. 471-478.
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