Electrospun fibers as a scaffolding platform for bone tissue repair

Seungyoun Lyu, Chunlan Huang, Hong Yang, Xinping Zhang

Research output: Contribution to journalArticlepeer-review


The purpose of the study is to investigate the effects of electrospun fiber diameter and orientation on differentiation and ECM organization of bone marrow stromal cells (BMSCs), in attempt to provide rationale for fabrication of a periosteum mimetic for bone defect repair. Cellular growth, differentiation, and ECM organization were analyzed on PLGA-based random and aligned fibers using fluorescent microscopy, gene analyses, electron scanning microscopy (SEM), and multiphoton laser scanning microscopy (MPLSM). BMSCs on aligned fibers had a reduced number of ALP+ colony at Day 10 as compared to the random fibers of the same size. However, the ALP+ area in the aligned fibers increased to a similar level as the random fibers at Day 21 following stimulation with osteogenic media. Compared with the random fibers, BMSCs on the aligned fibers showed a higher expression of OSX and RUNX2. Analyses of ECM on decellularized spun fibers showed highly organized ECM arranged according to the orientation of the spun fibers, with a broad size distribution of collagen fibers in a range of 40-2.4 μm. Taken together, our data support the use of submicron-sized electrospun fibers for engineering of oriented fibrous tissue mimetic, such as periosteum, for guided bone repair and reconstruction. © 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 31:1382-1389, 2013

Original languageEnglish (US)
Pages (from-to)1382-1389
Number of pages8
JournalJournal of Orthopaedic Research
Issue number9
StatePublished - Sep 2013
Externally publishedYes


  • electrospinning
  • extracellular matrix (ECM)
  • periosteum

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine


Dive into the research topics of 'Electrospun fibers as a scaffolding platform for bone tissue repair'. Together they form a unique fingerprint.

Cite this