Abstract
Electroporation-induced transformation of intact cells of Clostridium perfringens 3624A with plasmids pAMB1 and pHR106 resulted in 3.8 X 10(-5) and 4.2 X 10(-4) transformants per viable cell, respectively. With respect to shuttle plasmid pHR106, these values represent a greater than 100-fold increase in transformation frequency when compared with the values reported with polyethylene glycol-induced L-phase variants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2322-2324 |
| Number of pages | 3 |
| Journal | Applied and environmental microbiology |
| Volume | 54 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 1988 |
ASJC Scopus subject areas
- Biotechnology
- Food Science
- Applied Microbiology and Biotechnology
- Ecology