Electrophoretic separation and properties of winged bean seed trypsin inhibitor

David F. Hildebrand, Navagnana S. Hettiarachchy, Theodore Hymowitz, John W Erdman

Research output: Contribution to journalArticle

Abstract

Separation of the major trypsin inhibitor protein from crude extracts of winged bean seeds (WBTi) was accomplished using a cathodic polyacrylamide disc gel electrophoretic system. The molecular weight of the WBTi protein was determined to be about 20 000. Winged bean seed extracts showed trypsin‐inhibiting (Ti) activity similar to soya bean seed extracts, but large genotypic differences of the Ti activity in winged beans were found. Preliminary studies on the effects of processing on WBTi indicate that aqueous extracts of WBTi are at least as heat‐labile as the Ti of aqueous extracts of soya bean seeds. Autoclaving winged bean seed meal prior to protein extraction resulted in considerable reduction of protein solubility.

Original languageEnglish (US)
Pages (from-to)443-450
Number of pages8
JournalJournal of the Science of Food and Agriculture
Volume32
Issue number5
DOIs
StatePublished - May 1981

Fingerprint

Psophocarpus tetragonolobus
trypsin inhibitors
Seeds
seed extracts
seeds
extracts
beans
Proteins
autoclaving
proteins
protein solubility
polyacrylamide
Trypsin Inhibitors
Complex Mixtures
water
Solubility
gels
Meals
molecular weight
Molecular Weight

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics

Cite this

Electrophoretic separation and properties of winged bean seed trypsin inhibitor. / Hildebrand, David F.; Hettiarachchy, Navagnana S.; Hymowitz, Theodore; Erdman, John W.

In: Journal of the Science of Food and Agriculture, Vol. 32, No. 5, 05.1981, p. 443-450.

Research output: Contribution to journalArticle

Hildebrand, David F. ; Hettiarachchy, Navagnana S. ; Hymowitz, Theodore ; Erdman, John W. / Electrophoretic separation and properties of winged bean seed trypsin inhibitor. In: Journal of the Science of Food and Agriculture. 1981 ; Vol. 32, No. 5. pp. 443-450.
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