The synthesis of esterases was analysed in the ovariole of the milkweed bug using electrophoretic and histochemical methods. We found a total of 12 esterases from whole ovaries. Using the inhibitors p-hydroxymercuribenzoate, diisopropyl phosphorofluoridate and eserine, 3 of the bands were classed as acetylesterases, 7 carboxylesterases, and 2 arylesterases. No change in esterase patterns occurred during oogenesis. The esterase activity in the follicular epithelium was primarily arylesterase and carboxylesterase. A carboxylesterase was also identified from the central region of the follicle cell. The yolk cells contained mainly carboxylesterase. There was an apparent activation of enzyme activity following pre-treatment with eserine.
|Number of pages
|Wilhelm Roux' Archiv für Entwicklungsmechanik der Organismen
|Published - Sep 1972
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Developmental Biology
- Cell Biology