Electroosmotic flow in 'click' surface modified microfluidic channels

Shaurya Prakash, Timothy M. Long, Wan Jonathan, Jeffrey S. Moore, Mark A. Shannon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A rapid, facile, and modular surface modification scheme for the covalent attachment of pre-formed polymer moieties to self-assembled monolayers via 'click' chemistry within glass microfluidic channels (3 cm long, 110μm wide and 15 μm deep) is described. The effect that different moieties have on the electroosmotic flow (EOF) within the microchannels is evaluated. The application of linear polymers such as poly(ethylene glycol) (PEG) generates hydrophilic surfaces that reduce the analyte-wall interactions, thereby increasing separation efficiency and improving resolution, especially in bio-separations. Dendritic polymers such as poly(amido amine) (PAMAM) on channel walls can provide high-surface area structures with tunable surface charge depending on the generation of the dendrimer coating. Modified surfaces are characterized by X-ray photoelectron spectroscopy (XPS), Fourier Transform Infrared-Attenuated Total Reflection spectroscopy (FTIR-ATR), and contact angle measurements. EOF measurements in modified and unmodified channels provide information about wall-analyte interactions. A PAMAM dendrimer coated channel presents an amine terminated surface with a positive charge in contrast to a negatively charged bare-glass surface. Use of surface coatings can lead to an increase of the EOF by 15% as is the case for an azide terminated surface or reverse the direction of EOF as is the case for the PAMAM coatings by changing the surface charge polarity.

Original languageEnglish (US)
Title of host publicationProceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006
Pages249-256
Number of pages8
StatePublished - 2006
Event4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006 - Limerick, Ireland
Duration: Jun 19 2006Jun 21 2006

Publication series

NameProceedings of the 4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006
Volume2006 A

Other

Other4th International Conference on Nanochannels, Microchannels and Minichannels, ICNMM2006
CountryIreland
CityLimerick
Period6/19/066/21/06

ASJC Scopus subject areas

  • Engineering(all)

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