Electrochemical and spectrometric studies of double-strand calf thymus gland dna denatured by Al(III) at neutral pH

Fuping Zhang, Qing Cao, Jiongjia Cheng, Caihua Zhang, Na An, Shuping Bi

Research output: Contribution to journalArticle

Abstract

The interaction between double-strand calf thymus gland DNA (ds-DNA) and Al(III) was studied by using differential pulse voltammetry (DPV) at a hanging mercury drop electrode (HMDE), Raman spectrometry and circular dichroism (CD) spectra. It was shown that at neutral pH ds-DNA did not produce any cathodic peak at the HMDE in the potential window from -550 to -2000 mV vs. SCE. However, in the presence of Al(III), a cathodic peak was generated at about -1660 mV, which is ascribed to a reduction of adenine and cytosine residues of single denatured DNA (sd-DNA). It was concluded that ds-DNA was completely denatured to sd-DNA by Al(III) at a neutral pH. The apparent denaturing kinetic velocity constants of ds-DNA by Al(III) were derived from linear increases of the cathodic peak currents with time. When [Al(III)]×[OH] -3 ≥ 2 × 10- 26 , the precipitation of Al(OH) 3 was observed and identified by the Raman spectrum, and inductively coupled plasma atomic emission spectrometry (ICP-AES). CD spectra showed that the B-type of structure conformations of ds-DNA and related sd-DNA did not change with the increment of Al(III) from 5.0 × 10 -7 to 1.0 × 10 -5 M, but the corresponding absorption strengths increased. The related physiological significances and possible applications of the observations were considered. 2009

Original languageEnglish (US)
Pages (from-to)1019-1023
Number of pages5
JournalAnalytical Sciences
Volume25
Issue number8
DOIs
StatePublished - Aug 2009
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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