@article{3af1c10a8ffd43cf8b53cd19cf05fac5,
title = "Elasticity in Macrophage-Synthesized Biocrystals",
abstract = "Supramolecular crystalline assembly constitutes a rational approach to bioengineer intracellular structures. Here, biocrystals of clofazimine (CFZ) that form in vivo within macrophages were measured to have marked curvature. Isolated crystals, however, showed reduced curvature suggesting that intracellular forces bend these drug crystals. Consistent with the ability of biocrystals to elastically deform, the inherent crystal structure of the principal molecular component of the biocrystals—the hydrochloride salt of CFZ (CFZ-HCl)—has a corrugated packing along the (001) face and weak dispersive bonding in multiple directions. These characteristics were previously found to be linked to the elasticity of other organic crystals. Internal stress in bent CFZ-HCl led to photoelastic effects on the azimuthal orientation of polarized light transmittance. We propose that elastic, intracellular crystals can serve as templates to construct functional microdevices with different applications.",
keywords = "X-ray diffraction, biocrystals, clofazimine, crystal engineering, elasticity",
author = "Horstman, {Elizabeth M.} and Keswani, {Rahul K.} and Frey, {Benjamin A.} and Rzeczycki, {Phillip M.} and Vernon LaLone and Bertke, {Jeffery A.} and Kenis, {Paul J.A.} and Rosania, {Gus R.}",
note = "Funding Information: We thank the National Science Foundation (NSF) for a Graduate Research Fellowship to E.M.H. (DGE-1144245), National Institute of General Medical Sciences (NIGMS) for funding support to G.R.R. (R01GM078200), University of Michigan of Office of Research MCubed Program funding to G.R.R., Michigan Institute for Clinical & Health Research Pilot Seed funding to G.R.R. and R.K.K. (NIH: UL1TR000433) and the Interdisciplinary REU (Research Experiences for Undergraduates) Program housed in the College of Pharmacy, University of Michigan for funding B.A.F. through the NSF-Division of Biological Infrastructure (NSF-DBI) (DBI-1263079). We acknowledge the support of Dr. Gislaine Kuminek, Dr. Nair Rodriguez-Hornedo, Dr. Gi Sang Yoon and Sudha Sud (Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan) for help with DSC and animal experiments, Dr. Danielle Gray, George L. Clark (X-ray Facility and 3M Materials Laboratory, University of Illinois) for discussion about crystallographic data, and Lucas C. Gonzalez (Department of Chemical and Biomolecular Engineering, University of Illinois) for help with crystallization experiments. Publisher Copyright: {\textcopyright} 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2017",
month = feb,
day = "6",
doi = "10.1002/anie.201611195",
language = "English (US)",
volume = "56",
pages = "1815--1819",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley & Sons, Ltd.",
number = "7",
}