Efficient one-step synthesis of biologically related lariat RNAs by a deoxyribozyme

Yangming Wang, Scott K. Silverman

Research output: Contribution to journalArticle

Abstract

Closing the loop: The artificial 6BX22 deoxyribozyme (in green) synthesizes naturally occurring lariat RNAs in one step from readily available linear RNA substrates. The exquisite site selectivity of this macrocyclization reaction - which can form a 1597-membered ring in 72% yield with < 0.1 % side products - highlights the utility of catalytic DNA for transformations that are not possible with traditional synthetic methods. (Chemical Equation Presented).

Original languageEnglish (US)
Pages (from-to)5863-5866
Number of pages4
JournalAngewandte Chemie - International Edition
Volume44
Issue number36
DOIs
StatePublished - Sep 4 2005

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Keywords

  • DNA
  • Deoxyribozymes
  • Enzyme catalysis
  • Lariat RNA
  • Macrocycles

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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