Efficient delivery of a DNA aptamer-based biosensor into plant cells for glucose sensing through thiol-mediated uptake

Quanbing Mou, Xueyi Xue, Yuan Ma, Mandira Banik, Valeria Garcia, Weijie Guo, Jiang Wang, Tingjie Song, Li Qing Chen, Yi Lu

Research output: Contribution to journalArticlepeer-review

Abstract

DNA aptamers have been widely used as biosensors for detecting a variety of targets. Despite decades of success, they have not been applied to monitor any targets in plants, even though plants are a major platform for providing oxygen, food, and sustainable products ranging from energy fuels to chemicals, and high-value products such as pharmaceuticals. A major barrier to progress is a lack of efficient methods to deliver DNA into plant cells. We herein report a thiol-mediated uptake method that more efficiently delivers DNA into Arabidopsis and tobacco leaf cells than another state-of-the-art method, DNA nanostructures. Such a method allowed efficient delivery of a glucose DNA aptamer sensor into Arabidopsis for sensing glucose. This demonstration opens a new avenue to apply DNA aptamer sensors for functional studies of various targets, including metabolites, plant hormones, metal ions, and proteins in plants for a better understanding of the biodistribution and regulation of these species and their functions.

Original languageEnglish (US)
Article numbereabo0902
JournalScience Advances
Volume8
Issue number26
DOIs
StatePublished - Jul 2022

ASJC Scopus subject areas

  • General

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