TY - JOUR
T1 - Efficiency of antifreeze glycoproteins for cryopreservation of Nili-Ravi (Bubalus bubalis) buffalo bull sperm
AU - Qadeer, S.
AU - Khan, M. A.
AU - Ansari, M. S.
AU - Rakha, B. A.
AU - Ejaz, R.
AU - Iqbal, R.
AU - Younis, M.
AU - Ullah, N.
AU - DeVries, Arthur L.
AU - Akhter, S.
N1 - Funding Information:
The authors are thankful to Higher Education Commission Pakistan for providing financial assistance under 5000 Indigenous PhD Fellowship Program.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - Experiments were conducted to evaluate the effect of Antarctic fish antifreeze glycoproteins, (AFGP) size 1-5 (34-10.5kDa) and 7-8 (3.2 and 2.4kDa) in extender on buffalo bull sperm at cooling (4°C) and at post thawing. Semen was collected from three Nili-Ravi buffalo bulls with artificial vagina for 3 weeks. Qualifying ejaculates from each buffalo bull were diluted (at 37°C having 50×106sperm/mL) in tris-citric acid extender containing AFGP at 0 (control), 0.1, 1 and 10μg/mL. An aliquot of diluted semen was evaluated for sperm progressive motility and plasma membrane integrity, while the remaining fraction was cooled to 4°C in 2h. Further, an aliquot of cooled semen was evaluated for the previously described variables and the remaining fraction was cryopreserved (-196°C). After 24h of storage, straws were thawed at 37°C for 30s to assess post-thaw sperm quality. Inclusion of AFGP in the extender did not affect (P>0.05) sperm progressive motility and plasma membrane integrity of buffalo bull sperm at cooling stage (4°C). However, at post thawing, improvement (P<0.05) in sperm progressive motility and plasma membrane integrity was recorded in extender containing AFGP 1-5 and AFGP 7-8 at 1μg/mL compared to the control. Percentage of live sperm with an intact acrosome remained similar (P>0.05) in extenders containing different amounts of AFGP and control. In conclusion, supplementation of 1μg/ml of AFGP in extender improved the motility and plasma membrane integrity of Nili-Ravi buffalo sperm after thawing.
AB - Experiments were conducted to evaluate the effect of Antarctic fish antifreeze glycoproteins, (AFGP) size 1-5 (34-10.5kDa) and 7-8 (3.2 and 2.4kDa) in extender on buffalo bull sperm at cooling (4°C) and at post thawing. Semen was collected from three Nili-Ravi buffalo bulls with artificial vagina for 3 weeks. Qualifying ejaculates from each buffalo bull were diluted (at 37°C having 50×106sperm/mL) in tris-citric acid extender containing AFGP at 0 (control), 0.1, 1 and 10μg/mL. An aliquot of diluted semen was evaluated for sperm progressive motility and plasma membrane integrity, while the remaining fraction was cooled to 4°C in 2h. Further, an aliquot of cooled semen was evaluated for the previously described variables and the remaining fraction was cryopreserved (-196°C). After 24h of storage, straws were thawed at 37°C for 30s to assess post-thaw sperm quality. Inclusion of AFGP in the extender did not affect (P>0.05) sperm progressive motility and plasma membrane integrity of buffalo bull sperm at cooling stage (4°C). However, at post thawing, improvement (P<0.05) in sperm progressive motility and plasma membrane integrity was recorded in extender containing AFGP 1-5 and AFGP 7-8 at 1μg/mL compared to the control. Percentage of live sperm with an intact acrosome remained similar (P>0.05) in extenders containing different amounts of AFGP and control. In conclusion, supplementation of 1μg/ml of AFGP in extender improved the motility and plasma membrane integrity of Nili-Ravi buffalo sperm after thawing.
KW - Antifreeze glycoproteins
KW - Buffalo bull sperm
KW - Ice crystal
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U2 - 10.1016/j.anireprosci.2015.03.015
DO - 10.1016/j.anireprosci.2015.03.015
M3 - Article
C2 - 25863987
AN - SCOPUS:84928823489
VL - 157
SP - 56
EP - 62
JO - Animal Reproduction Science
JF - Animal Reproduction Science
SN - 0378-4320
ER -