Effects of voluntary wheel running on LPS-induced sickness behavior in aged mice

Stephen A. Martin, Brandt D. Pence, Ryan M. Greene, Stephanie J. Johnson, Robert Dantzer, Keith W. Kelley, Jeffrey A. Woods

Research output: Contribution to journalArticle

Abstract

Peripheral stimulation of the innate immune system with LPS causes exaggerated neuroinflammation and prolonged sickness behavior in aged mice. Regular moderate intensity exercise has been shown to exert anti-inflammatory effects that may protect against inappropriate neuroinflammation and sickness in aged mice. The purpose of this study was to test the hypothesis that voluntary wheel running would attenuate LPS-induced sickness behavior and proinflammatory cytokine gene expression in ∼22-month-old C57BL/6. J mice. Mice were housed with a running wheel (VWR), locked-wheel (Locked), or no wheel (Standard) for 10. weeks, after which they were intraperitoneally injected with LPS across a range of doses (0.02, 0.08, 0.16, 0.33. mg/kg). VWR mice ran on average 3.5. km/day and lost significantly more body weight and body fat, and increased their forced exercise tolerance compared to Locked and Shoebox mice. VWR had no effect on LPS-induced anorexia, adipsia, weight-loss, or reductions in locomotor activity at any LPS dose when compared to Locked and Shoebox groups. LPS induced sickness behavior in a dose-dependent fashion (0.33. >. 0.02. mg/kg). Twenty-four hours post-injection (0.33. mg/kg LPS or Saline) we found a LPS-induced upregulation of whole brain TNFα, IL-1β, and IL-10 mRNA, and increased IL-1β and IL-6 in the spleen and liver; these effects were not attenuated by VWR. We conclude that VWR does not reduce LPS-induced exaggerated or prolonged sickness behavior in aged animals, or 24. h post-injection (0.33. mg/kg LPS or Saline) brain and peripheral proinflammatory cytokine gene expression. The necessity of the sickness response is critical for survival and may outweigh the subtle benefits of exercise training in aged animals.

Original languageEnglish (US)
Pages (from-to)113-123
Number of pages11
JournalBrain, Behavior, and Immunity
Volume29
DOIs
StatePublished - Mar 1 2013

Fingerprint

Illness Behavior
Running
Interleukin-1
Weight Loss
Cytokines
Gene Expression
Injections
Exercise Tolerance
Brain
Anorexia
Locomotion
Interleukin-10
Adipose Tissue
Immune System
Interleukin-6
Anti-Inflammatory Agents
Up-Regulation
Spleen
Body Weight
Exercise

Keywords

  • Aging
  • Exercise
  • Inflammation
  • Sickness behavior

ASJC Scopus subject areas

  • Immunology
  • Endocrine and Autonomic Systems
  • Behavioral Neuroscience

Cite this

Martin, S. A., Pence, B. D., Greene, R. M., Johnson, S. J., Dantzer, R., Kelley, K. W., & Woods, J. A. (2013). Effects of voluntary wheel running on LPS-induced sickness behavior in aged mice. Brain, Behavior, and Immunity, 29, 113-123. https://doi.org/10.1016/j.bbi.2012.12.014

Effects of voluntary wheel running on LPS-induced sickness behavior in aged mice. / Martin, Stephen A.; Pence, Brandt D.; Greene, Ryan M.; Johnson, Stephanie J.; Dantzer, Robert; Kelley, Keith W.; Woods, Jeffrey A.

In: Brain, Behavior, and Immunity, Vol. 29, 01.03.2013, p. 113-123.

Research output: Contribution to journalArticle

Martin, Stephen A. ; Pence, Brandt D. ; Greene, Ryan M. ; Johnson, Stephanie J. ; Dantzer, Robert ; Kelley, Keith W. ; Woods, Jeffrey A. / Effects of voluntary wheel running on LPS-induced sickness behavior in aged mice. In: Brain, Behavior, and Immunity. 2013 ; Vol. 29. pp. 113-123.
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