Effects of triclosan and triclocarban on denitrification and N2O emissions in paddy soil

Shuntao Chen, Joanne C. Chee-Sanford, Wendy Yang, Robert A Sanford, Jianqiu Chen, Xiaoyuan Yan, Jun Shan

Research output: Contribution to journalArticle

Abstract

Triclosan (TCS) and triclocarban (TCC) are two common antimicrobial compounds, which are widely used as ingredients in pharmaceuticals and personal care products. They occur ubiquitously in soil due to biosolid application as agricultural fertilizers, but their influence on microbially mediated soil biogeochemical processes is poorly understood. We tested the effects of varying concentrations of TCS and TCC applied both individually and together on denitrification and N2O emissions in paddy soil. We also quantified denitrification functional gene abundances by q-PCR to elucidate the microbial mechanisms of TCS and TCC's effects. Our results showed that TCS and TCC exposure both individually and together significantly (p < 0.05) inhibited denitrification (7.0–36.7%) and N2O emissions (15.4–86.4%) except for the 0.01 mg kg−1 TCC treatment in which denitrification was slightly but significantly (p < 0.05) stimulated. The inhibitory effects of TCS and TCC exposure were mainly attributed to their negative net effects on denitrifying bacteria as suggested by the decrease in abundances of 16S rRNA, narG, nirK and clade I nosZ genes in the TCS and TCC treatments. Overall, we found that TCS and TCC exposure in paddy soil could substantially alter nitrogen cycling in rice paddy ecosystems by inhibiting denitrification and N2O emissions. These effects should be taken into consideration when evaluating the environmental impacts of TCS and TCC.

Original languageEnglish (US)
Article number133782
JournalScience of the Total Environment
Volume695
DOIs
StatePublished - Dec 10 2019

Fingerprint

Triclosan
Denitrification
denitrification
Soils
soil
Genes
Biosolids
gene
biosolid
Fertilizers
Drug products
Ecosystems
Environmental impact
Bacteria
environmental impact
rice
triclocarban
effect
fertilizer
Nitrogen

Keywords

  • Denitrification rate
  • N tracer
  • NO emission
  • Paddy soil
  • Triclocarban
  • Triclosan

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

Cite this

Effects of triclosan and triclocarban on denitrification and N2O emissions in paddy soil. / Chen, Shuntao; Chee-Sanford, Joanne C.; Yang, Wendy; Sanford, Robert A; Chen, Jianqiu; Yan, Xiaoyuan; Shan, Jun.

In: Science of the Total Environment, Vol. 695, 133782, 10.12.2019.

Research output: Contribution to journalArticle

Chen, Shuntao ; Chee-Sanford, Joanne C. ; Yang, Wendy ; Sanford, Robert A ; Chen, Jianqiu ; Yan, Xiaoyuan ; Shan, Jun. / Effects of triclosan and triclocarban on denitrification and N2O emissions in paddy soil. In: Science of the Total Environment. 2019 ; Vol. 695.
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AB - Triclosan (TCS) and triclocarban (TCC) are two common antimicrobial compounds, which are widely used as ingredients in pharmaceuticals and personal care products. They occur ubiquitously in soil due to biosolid application as agricultural fertilizers, but their influence on microbially mediated soil biogeochemical processes is poorly understood. We tested the effects of varying concentrations of TCS and TCC applied both individually and together on denitrification and N2O emissions in paddy soil. We also quantified denitrification functional gene abundances by q-PCR to elucidate the microbial mechanisms of TCS and TCC's effects. Our results showed that TCS and TCC exposure both individually and together significantly (p < 0.05) inhibited denitrification (7.0–36.7%) and N2O emissions (15.4–86.4%) except for the 0.01 mg kg−1 TCC treatment in which denitrification was slightly but significantly (p < 0.05) stimulated. The inhibitory effects of TCS and TCC exposure were mainly attributed to their negative net effects on denitrifying bacteria as suggested by the decrease in abundances of 16S rRNA, narG, nirK and clade I nosZ genes in the TCS and TCC treatments. Overall, we found that TCS and TCC exposure in paddy soil could substantially alter nitrogen cycling in rice paddy ecosystems by inhibiting denitrification and N2O emissions. These effects should be taken into consideration when evaluating the environmental impacts of TCS and TCC.

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