TY - JOUR
T1 - Effects of syringe type and storage conditions on results of equine blood gas and acid-base analysis
AU - Kennedy, Sarah A.
AU - Constable, Peter D.
AU - Sen, Ismail
AU - Couëtil, Laurent
PY - 2012/7
Y1 - 2012/7
N2 - Objective-To determine effects of syringe type and storage conditions on blood gas and acid-base values for equine blood samples. Sample-Blood samples obtained from 8 healthy horses. Procedures-Heparinized jugular venous blood was equilibrated via a tonometer at 37°C with 12% O2 and 5% CO2. Aliquots (3 mL) of tonometer-equilibrated blood were collected in random order by use of a glass syringe (GS), general-purpose polypropylene syringe (GPPS), or polypropylene syringe designed for blood gas analysis (PSBGA) and stored in ice water (0°C) or at room temperature (22°C) for 0, 5, 15, 30, 60, or 120 minutes. Blood pH was measured, and blood gas analysis was performed; data were analyzed by use of multivariable regression analysis. Results-Blood Po2 remained constant for the reference method (GS stored at 0°C) but decreased linearly at a rate of 7.3 mm Hg/h when stored in a GS at 22°C. In contrast, Po2 increased when blood was stored at 0°C in a GPPS and PSBGA or at 22°C in a GPPS; however, Po2 did not change when blood was stored at 22°C in a PSBGA. Calculated values for plasma concentration of HCO3 and total CO2 concentration remained constant in the 3 syringe types when blood was stored at 22°C for 2 hours but increased when blood was stored in a GS or GPPS at 0°C. Conclusions and Clinical Relevance-Blood samples for blood gas and acid-base analysis should be collected into a GS and stored at 0°C or collected into a PSBGA and stored at room temperature.
AB - Objective-To determine effects of syringe type and storage conditions on blood gas and acid-base values for equine blood samples. Sample-Blood samples obtained from 8 healthy horses. Procedures-Heparinized jugular venous blood was equilibrated via a tonometer at 37°C with 12% O2 and 5% CO2. Aliquots (3 mL) of tonometer-equilibrated blood were collected in random order by use of a glass syringe (GS), general-purpose polypropylene syringe (GPPS), or polypropylene syringe designed for blood gas analysis (PSBGA) and stored in ice water (0°C) or at room temperature (22°C) for 0, 5, 15, 30, 60, or 120 minutes. Blood pH was measured, and blood gas analysis was performed; data were analyzed by use of multivariable regression analysis. Results-Blood Po2 remained constant for the reference method (GS stored at 0°C) but decreased linearly at a rate of 7.3 mm Hg/h when stored in a GS at 22°C. In contrast, Po2 increased when blood was stored at 0°C in a GPPS and PSBGA or at 22°C in a GPPS; however, Po2 did not change when blood was stored at 22°C in a PSBGA. Calculated values for plasma concentration of HCO3 and total CO2 concentration remained constant in the 3 syringe types when blood was stored at 22°C for 2 hours but increased when blood was stored in a GS or GPPS at 0°C. Conclusions and Clinical Relevance-Blood samples for blood gas and acid-base analysis should be collected into a GS and stored at 0°C or collected into a PSBGA and stored at room temperature.
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U2 - 10.2460/ajvr.73.7.979
DO - 10.2460/ajvr.73.7.979
M3 - Article
C2 - 22738049
AN - SCOPUS:84863435055
SN - 0002-9645
VL - 73
SP - 979
EP - 987
JO - American journal of veterinary research
JF - American journal of veterinary research
IS - 7
ER -