Effects of salts on internal DNA pressure and mechanical properties of phage capsids

Alex Evilevitch; Wouter H. Roos; Irena L. Ivanovska; Meerim Jeembaeva; Bengt Jönsson; Gijs J.L. Wuite

doi:10.1016/j.jmb.2010.10.039

Effects of salts on internal DNA pressure and mechanical properties of phage capsids

Alex Evilevitch, Wouter H. Roos, Irena L. Ivanovska, Meerim Jeembaeva, Bengt Jönsson, Gijs J.L. Wuite

Research output: Contribution to journal › Article › peer-review

Abstract

Based on atomic force microscopy nanoindentation measurements of phage λ, we previously proposed a minimal model describing the effect of water hydrating DNA that strengthens viral capsids against external deformation at wild-type DNA packing density. Here, we report proof of this model by testing the prediction that DNA hydration forces can be dramatically decreased by addition of multivalent ions (Mg²⁺ and Sp⁴⁺). These results are explained using a DNA hydration model without adjustable parameters. The model also predicts the stiffness of other DNA-filled capsids, which we confirm using bacteriophage φ29 and herpes simplex virus type 1 particles.

Original language	English (US)
Pages (from-to)	18-23
Number of pages	6
Journal	Journal of Molecular Biology
Volume	405
Issue number	1
DOIs	https://doi.org/10.1016/j.jmb.2010.10.039
State	Published - Jan 7 2011
Externally published	Yes

Keywords

AFM
bacteriophage ?
genome ejection
nanoindentation
stability

ASJC Scopus subject areas

Structural Biology
Molecular Biology

Online availability

10.1016/j.jmb.2010.10.039

Library availability

Discover UIUC Full Text

Cite this

@article{e5ec03145b7b4119a068f88fc8b64e83,

title = "Effects of salts on internal DNA pressure and mechanical properties of phage capsids",

abstract = "Based on atomic force microscopy nanoindentation measurements of phage λ, we previously proposed a minimal model describing the effect of water hydrating DNA that strengthens viral capsids against external deformation at wild-type DNA packing density. Here, we report proof of this model by testing the prediction that DNA hydration forces can be dramatically decreased by addition of multivalent ions (Mg2+ and Sp4+). These results are explained using a DNA hydration model without adjustable parameters. The model also predicts the stiffness of other DNA-filled capsids, which we confirm using bacteriophage φ29 and herpes simplex virus type 1 particles.",

keywords = "AFM, bacteriophage ?, genome ejection, nanoindentation, stability",

author = "Alex Evilevitch and Roos, {Wouter H.} and Ivanovska, {Irena L.} and Meerim Jeembaeva and Bengt J{\"o}nsson and Wuite, {Gijs J.L.}",

note = "Funding Information: This work was supported by the Foundation for Fundamental Research on Matter (FOM), by a Vernieuwingsimpuls and a VICI grant from the Netherlands Organization for Scientific Research (to G.J.W.), and by a European Laser Center grant and the Swedish Research Council (to A.E.). We would like to thank J. L. Carrascosa for providing mature bacteriophage ϕ29 particles. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2011",

month = jan,

day = "7",

doi = "10.1016/j.jmb.2010.10.039",

language = "English (US)",

volume = "405",

pages = "18--23",

journal = "Journal of Molecular Biology",

issn = "0022-2836",

publisher = "Academic Press",

number = "1",

}

TY - JOUR

T1 - Effects of salts on internal DNA pressure and mechanical properties of phage capsids

AU - Evilevitch, Alex

AU - Roos, Wouter H.

AU - Ivanovska, Irena L.

AU - Jeembaeva, Meerim

AU - Jönsson, Bengt

AU - Wuite, Gijs J.L.

N1 - Funding Information: This work was supported by the Foundation for Fundamental Research on Matter (FOM), by a Vernieuwingsimpuls and a VICI grant from the Netherlands Organization for Scientific Research (to G.J.W.), and by a European Laser Center grant and the Swedish Research Council (to A.E.). We would like to thank J. L. Carrascosa for providing mature bacteriophage ϕ29 particles. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2011/1/7

Y1 - 2011/1/7

N2 - Based on atomic force microscopy nanoindentation measurements of phage λ, we previously proposed a minimal model describing the effect of water hydrating DNA that strengthens viral capsids against external deformation at wild-type DNA packing density. Here, we report proof of this model by testing the prediction that DNA hydration forces can be dramatically decreased by addition of multivalent ions (Mg2+ and Sp4+). These results are explained using a DNA hydration model without adjustable parameters. The model also predicts the stiffness of other DNA-filled capsids, which we confirm using bacteriophage φ29 and herpes simplex virus type 1 particles.

AB - Based on atomic force microscopy nanoindentation measurements of phage λ, we previously proposed a minimal model describing the effect of water hydrating DNA that strengthens viral capsids against external deformation at wild-type DNA packing density. Here, we report proof of this model by testing the prediction that DNA hydration forces can be dramatically decreased by addition of multivalent ions (Mg2+ and Sp4+). These results are explained using a DNA hydration model without adjustable parameters. The model also predicts the stiffness of other DNA-filled capsids, which we confirm using bacteriophage φ29 and herpes simplex virus type 1 particles.

KW - AFM

KW - bacteriophage ?

KW - genome ejection

KW - nanoindentation

KW - stability

UR - http://www.scopus.com/inward/record.url?scp=78650420324&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78650420324&partnerID=8YFLogxK

U2 - 10.1016/j.jmb.2010.10.039

DO - 10.1016/j.jmb.2010.10.039

M3 - Article

C2 - 21035458

AN - SCOPUS:78650420324

SN - 0022-2836

VL - 405

SP - 18

EP - 23

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

IS - 1

ER -

Effects of salts on internal DNA pressure and mechanical properties of phage capsids

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this