Effects of salts on internal DNA pressure and mechanical properties of phage capsids

Alex Evilevitch, Wouter H. Roos, Irena L. Ivanovska, Meerim Jeembaeva, Bengt Jönsson, Gijs J.L. Wuite

Research output: Contribution to journalArticle

Abstract

Based on atomic force microscopy nanoindentation measurements of phage λ, we previously proposed a minimal model describing the effect of water hydrating DNA that strengthens viral capsids against external deformation at wild-type DNA packing density. Here, we report proof of this model by testing the prediction that DNA hydration forces can be dramatically decreased by addition of multivalent ions (Mg2+ and Sp4+). These results are explained using a DNA hydration model without adjustable parameters. The model also predicts the stiffness of other DNA-filled capsids, which we confirm using bacteriophage φ29 and herpes simplex virus type 1 particles.

Original languageEnglish (US)
Pages (from-to)18-23
Number of pages6
JournalJournal of Molecular Biology
Volume405
Issue number1
DOIs
StatePublished - Jan 7 2011

Keywords

  • AFM
  • bacteriophage ?
  • genome ejection
  • nanoindentation
  • stability

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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  • Cite this

    Evilevitch, A., Roos, W. H., Ivanovska, I. L., Jeembaeva, M., Jönsson, B., & Wuite, G. J. L. (2011). Effects of salts on internal DNA pressure and mechanical properties of phage capsids. Journal of Molecular Biology, 405(1), 18-23. https://doi.org/10.1016/j.jmb.2010.10.039