TY - JOUR
T1 - Effects of Intra-Amniotic Administration of the Hydrolyzed Protein of Chia (Salvia hispanica L.) and Lacticaseibacillus paracasei on Intestinal Functionality, Morphology, and Bacterial Populations, In Vivo (Gallus gallus)
AU - Mishima, Marcella Duarte Villas
AU - Martino, Hércia Stampini Duarte
AU - Kolba, Nikolai
AU - Shah, Drashti Dhirenkumar
AU - Grancieri, Mariana
AU - Dos Santos, Karina Maria Olbrich
AU - Lima, Janine Passos
AU - Da Silva, Bárbara Pereira
AU - Gonzalez de Mejia, Elvira
AU - Tako, Elad
N1 - The authors thank the Coordination for the Improvement of Higher Educational Personnel (CAPES), Brazil, for providing the scholarship support in the Capes-Print Program (process number 88887.694292/2022-00), and the National Council of Technological and Scientific Development (CNPq, Brazil) for the Research Productivity fellowships [PQ2—grant number 310910/2020-0]. Additionally, they thank EMBRAPA (Brazilian Agricultural Research Corporation, SEG 20.20.03.054.00.00), Rio de Janeiro, Brazil. All authors have consented to all the acknowledgements included here.
PY - 2023/4
Y1 - 2023/4
N2 - As a protein source, chia contains high concentrations of bioactive peptides. Probiotics support a healthy digestive tract and immune system. Our study evaluated the effects of the intra-amniotic administration of the hydrolyzed chia protein and the probiotic Lacticaseibacillus paracasei on intestinal bacterial populations, the intestinal barrier, the inflammatory response, and brush border membrane functionality in ovo (Gallus gallus). Fertile broiler (Gallus gallus) eggs (n = 9/group) were divided into 5 groups: (NI) non-injected; (H2O) 18 MΩ H2O; (CP) 10 mg/mL hydrolyzed chia protein; (CPP) 10 mg/mL hydrolyzed chia protein + 106 colony-forming unit (CFU) L. paracasei; (P) 106 CFU L. paracasei. The intra-amniotic administration was performed on day 17 of incubation. At hatching (day 21), the animals were euthanized, and the duodenum and cecum content were collected. The probiotic downregulated the gene expression of NF-κβ, increased Lactobacillus and E. coli, and reduced Clostridium populations. The hydrolyzed chia protein downregulated the gene expression of TNF-α, increased OCLN, MUC2, and aminopeptidase, reduced Bifidobacterium, and increased Lactobacillus. The three experimental groups improved in terms of intestinal morphology. The current results suggest that the intra-amniotic administration of the hydrolyzed chia protein or a probiotic promoted positive changes in terms of the intestinal inflammation, barrier, and morphology, improving intestinal health.
AB - As a protein source, chia contains high concentrations of bioactive peptides. Probiotics support a healthy digestive tract and immune system. Our study evaluated the effects of the intra-amniotic administration of the hydrolyzed chia protein and the probiotic Lacticaseibacillus paracasei on intestinal bacterial populations, the intestinal barrier, the inflammatory response, and brush border membrane functionality in ovo (Gallus gallus). Fertile broiler (Gallus gallus) eggs (n = 9/group) were divided into 5 groups: (NI) non-injected; (H2O) 18 MΩ H2O; (CP) 10 mg/mL hydrolyzed chia protein; (CPP) 10 mg/mL hydrolyzed chia protein + 106 colony-forming unit (CFU) L. paracasei; (P) 106 CFU L. paracasei. The intra-amniotic administration was performed on day 17 of incubation. At hatching (day 21), the animals were euthanized, and the duodenum and cecum content were collected. The probiotic downregulated the gene expression of NF-κβ, increased Lactobacillus and E. coli, and reduced Clostridium populations. The hydrolyzed chia protein downregulated the gene expression of TNF-α, increased OCLN, MUC2, and aminopeptidase, reduced Bifidobacterium, and increased Lactobacillus. The three experimental groups improved in terms of intestinal morphology. The current results suggest that the intra-amniotic administration of the hydrolyzed chia protein or a probiotic promoted positive changes in terms of the intestinal inflammation, barrier, and morphology, improving intestinal health.
KW - gut health
KW - microbiota
KW - intestinal barrier
KW - inflammation
KW - chia seed
KW - bioactive peptides
KW - in vivo
KW - probiotic
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UR - http://www.scopus.com/inward/citedby.url?scp=85156176474&partnerID=8YFLogxK
U2 - 10.3390/nu15081831
DO - 10.3390/nu15081831
M3 - Article
C2 - 37111052
SN - 2072-6643
VL - 15
JO - Nutrients
JF - Nutrients
IS - 8
M1 - 1831
ER -