Effects of exercise and dietary epigallocatechin gallate and β-alanine on skeletal muscle in aged mice

Brandt D. Pence, Trisha E. Gibbons, Tushar K. Bhattacharya, Houston Mach, Jessica M. Ossyra, Geraldine Petr, Stephen A. Martin, Lin Wang, Stanislav S. Rubakhin, Jonathan V. Sweedler, Robert H. McCusker, Keith W. Kelley, Justin S. Rhodes, Rodney W. Johnson, Jeffrey A. Woods

Research output: Contribution to journalArticle

Abstract

Aging leads to sarcopenia and loss of physical function. We examined whether voluntary wheel running, when combined with dietary supplementation with (−)-epigallocatechin-3-gallate (EGCG) and β-alanine (β-ALA), could improve muscle function and alter gene expression in the gastrocnemius of aged mice. Seventeen-month-old BALB/cByJ mice were given access to a running wheel or remained sedentary for 41 days while receiving either AIN-93M (standard feed) or AIN-93M containing 1.5 mg·kg−1 EGCG and 3.43 mg·kg−1 β-ALA. Mice underwent tests over 11 days from day 29 to day 39 of the study period, including muscle function testing (grip strength, treadmill exhaustive fatigue, rotarod). Following a rest day, mice were euthanized and gastrocnemii were collected for analysis of gene expression by quantitative PCR. Voluntary wheel running (VWR) improved rotarod and treadmill exhaustive fatigue performance and maintained grip strength in aged mice, while dietary intervention had no effect. VWR increased gastrocnemius expression of several genes, including those encoding interleukin-6 (Il6, p = 0.001), superoxide dismutase 1 (Sod1, p = 0.046), peroxisome proliferator-activated receptor gamma coactivator 1-(Ppargc1a, p = 0.013), forkhead box protein O3 (Foxo3, p = 0.005), and brain-derived neurotrophic factor (Bdnf, p = 0.008), while reducing gastrocnemius levels of the lipid peroxidation marker 4-hydroxynonenal (p = 0.019). Dietary intervention alone increased gastrocnemius expression of Ppargc1a (p = 0.033) and genes encoding NAD-dependent protein deacetylase sirtuin-1 (Sirt1, p = 0.039), insulin-like growth factor I (Igf1, p = 0.003), and macrophage marker CD11b (Itgam, p = 0.016). Exercise and a diet containing β-ALA and EGCG differentially regulated gene expression in the gastrocnemius of aged mice, while VWR but not dietary intervention improved muscle function. We found no synergistic effects between dietary intervention and VWR.

Original languageEnglish (US)
Pages (from-to)181-190
Number of pages10
JournalApplied Physiology, Nutrition and Metabolism
Volume41
Issue number2
DOIs
StatePublished - Oct 26 2015

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Running
Alanine
Skeletal Muscle
Gene Expression
Hand Strength
Muscles
Fatigue
Sirtuin 1
Sarcopenia
Brain-Derived Neurotrophic Factor
Dietary Supplements
Insulin-Like Growth Factor I
NAD
Lipid Peroxidation
epigallocatechin gallate
Interleukin-6
Macrophages
Diet
Polymerase Chain Reaction
Genes

Keywords

  • Aging
  • Animal model
  • Gene expression
  • Muscle function
  • Supplementation
  • Voluntary wheel running

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Nutrition and Dietetics
  • Physiology (medical)

Cite this

Effects of exercise and dietary epigallocatechin gallate and β-alanine on skeletal muscle in aged mice. / Pence, Brandt D.; Gibbons, Trisha E.; Bhattacharya, Tushar K.; Mach, Houston; Ossyra, Jessica M.; Petr, Geraldine; Martin, Stephen A.; Wang, Lin; Rubakhin, Stanislav S.; Sweedler, Jonathan V.; McCusker, Robert H.; Kelley, Keith W.; Rhodes, Justin S.; Johnson, Rodney W.; Woods, Jeffrey A.

In: Applied Physiology, Nutrition and Metabolism, Vol. 41, No. 2, 26.10.2015, p. 181-190.

Research output: Contribution to journalArticle

Pence, Brandt D. ; Gibbons, Trisha E. ; Bhattacharya, Tushar K. ; Mach, Houston ; Ossyra, Jessica M. ; Petr, Geraldine ; Martin, Stephen A. ; Wang, Lin ; Rubakhin, Stanislav S. ; Sweedler, Jonathan V. ; McCusker, Robert H. ; Kelley, Keith W. ; Rhodes, Justin S. ; Johnson, Rodney W. ; Woods, Jeffrey A. / Effects of exercise and dietary epigallocatechin gallate and β-alanine on skeletal muscle in aged mice. In: Applied Physiology, Nutrition and Metabolism. 2015 ; Vol. 41, No. 2. pp. 181-190.
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AU - Mach, Houston

AU - Ossyra, Jessica M.

AU - Petr, Geraldine

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AU - Wang, Lin

AU - Rubakhin, Stanislav S.

AU - Sweedler, Jonathan V.

AU - McCusker, Robert H.

AU - Kelley, Keith W.

AU - Rhodes, Justin S.

AU - Johnson, Rodney W.

AU - Woods, Jeffrey A.

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N2 - Aging leads to sarcopenia and loss of physical function. We examined whether voluntary wheel running, when combined with dietary supplementation with (−)-epigallocatechin-3-gallate (EGCG) and β-alanine (β-ALA), could improve muscle function and alter gene expression in the gastrocnemius of aged mice. Seventeen-month-old BALB/cByJ mice were given access to a running wheel or remained sedentary for 41 days while receiving either AIN-93M (standard feed) or AIN-93M containing 1.5 mg·kg−1 EGCG and 3.43 mg·kg−1 β-ALA. Mice underwent tests over 11 days from day 29 to day 39 of the study period, including muscle function testing (grip strength, treadmill exhaustive fatigue, rotarod). Following a rest day, mice were euthanized and gastrocnemii were collected for analysis of gene expression by quantitative PCR. Voluntary wheel running (VWR) improved rotarod and treadmill exhaustive fatigue performance and maintained grip strength in aged mice, while dietary intervention had no effect. VWR increased gastrocnemius expression of several genes, including those encoding interleukin-6 (Il6, p = 0.001), superoxide dismutase 1 (Sod1, p = 0.046), peroxisome proliferator-activated receptor gamma coactivator 1-(Ppargc1a, p = 0.013), forkhead box protein O3 (Foxo3, p = 0.005), and brain-derived neurotrophic factor (Bdnf, p = 0.008), while reducing gastrocnemius levels of the lipid peroxidation marker 4-hydroxynonenal (p = 0.019). Dietary intervention alone increased gastrocnemius expression of Ppargc1a (p = 0.033) and genes encoding NAD-dependent protein deacetylase sirtuin-1 (Sirt1, p = 0.039), insulin-like growth factor I (Igf1, p = 0.003), and macrophage marker CD11b (Itgam, p = 0.016). Exercise and a diet containing β-ALA and EGCG differentially regulated gene expression in the gastrocnemius of aged mice, while VWR but not dietary intervention improved muscle function. We found no synergistic effects between dietary intervention and VWR.

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