Effects of ethanol feeding on liver, kidney and jejunal membranes of micropigs

Jesús Villanueva, Carol J. Chandler, Nell Shimasaki, Anna B. Tang, Manabu Nakamura, Stephen D. Phinney, Charles H. Halsted

Research output: Contribution to journalArticlepeer-review

Abstract

The micropig model of chronic alcoholism was used to study the relationship of lipid composition and physical properties in three different tissue membranes from the same animals. Ethanol feeding reduced membrane anisotropy, as measured with the diphenylhexatriene probe, in liver plasma and kidney brush‐border membranes but not in jejunal brushborder membranes. Preincubation with ethanol reduced anisotropy in each of the three control membranes, whereas all three membranes from the ethanol‐fed group were relatively tolerant to the acute effect of ethanol. In liver and kidney membranes, ethanol feeding increased levels of linoleic (18:2ω6) acid and decreased levels of arachidonic (20:4ω6) and docosahexaenoic (22:6ω3) acids and their specific double‐bond positions, consistent with reduced activities of Δ6 and Δ5 fatty acid desaturases. In liver and kidney membranes, anisotropy parameters and the acute effect of ethanol correlated inversely with levels of linoleic acid and directly with levels of arachidonic and docosahexaenoic acids and their specific double bonds. Levels of docosahexaenoic acid correlated with the acute effect of ethanol in all three membranes. Phospholipid fatty acid profiles were similar in jejunal brush‐border membranes and terminal bile samples, suggesting that the effects of ethanol on jejunal fatty acids and physical properties are modulated by intraluminal biliary phospholipids. The effect of ethanol on anisotropy could not be attributed to changes in membrane cholesterol/phospholipid ratios. These studies affirm the value of this new animal model of chronic alcoholism and provide comprehensive evidence for the central role of fatty acid desaturation in the membrane‐associated effects of ethanol exposure. (HEPATOLOGY 1994;19:1229–1240.)

Original languageEnglish (US)
Pages (from-to)1229-1240
Number of pages12
JournalHepatology
Volume19
Issue number5
DOIs
StatePublished - May 1994
Externally publishedYes

ASJC Scopus subject areas

  • Hepatology

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