Effects of estradiol and progesterone on plasma gonadotropins, prolactin, and LHRH in specific brain areas of ovariectomized rats

Phyllis M Wise, P. Camp-Grossman, C. A. Barraclough

    Research output: Contribution to journalArticle

    Abstract

    The authors examined the effects of physiological concentrations of estradiol (E2) and progesterone (P) on plasma LH, FSH, and prolactin (PRL) and on LHRH concentrations in microdissected brain regions in ovariectomized (OVX) rats. One week after ovariectomy (Day 0), rats received Silastic capsules containing only sesame oil or 37.5, 75.0 or 150 μg E2/ml of oil s.c. The E2 capsules produced plasma estrogen concentrations of 7.0, 9.6, or 15.4 pg/ml, respectively, on Day 2 whereas oil-treated controls had 5.4 pg/ml of E2 in plasma. All three E2-treated groups of rats had LH surges of comparable magnitude during the afternoon of Day 2. Two Silastic capsules of P (50 mg/ml) were implanted at 0900 h on Day 2 into E2-treated rats. In animals in which E2 levels were 7.0 or 9.6 pg/ml, P only moderately amplified the LH surges. However, when plasma E2 concentrations reached 15 pg/ml, P treatment evoked a massive release of LH and advanced the time of release by 1 h. In contrast, the stress of inserting oil capsules into ether-anesthetized E2-treated rats at 0900 h on Day 2 had no effect on afternoon LH surges in these animals. FSH surges occurred only in rats receiving both E2 and P. PRL surges were induced using the highest concentration of E2 and were advanced in time by P. When the highest dose of E2 was used, LHRH concentrations in the median eminence (ME) increased prior to and decreased during the E2 P-induced surge. These changes were not paralleled by any changes in the other anterior brain regions we examined (suprachiasmatic preoptic nucleus, suprachiasmatic nucleus, medial preoptic nucleus, anterior hypothalamic nucleus, and retrochiasmatic area). No changes in LHRH concentrations were observed under any other steroid treatment regimen. Higher plasma E2 concentrations induced by the 150 μg/ml E2 capsule were correlated with higher LHRH concentrations in the median eminence concomitant with a parallel trend in some of the anterior brain areas.

    Original languageEnglish (US)
    Pages (from-to)820-830
    Number of pages11
    JournalBiology of reproduction
    Volume24
    Issue number4
    DOIs
    StatePublished - Jan 1 1981

    Fingerprint

    Gonadotropins
    Gonadotropin-Releasing Hormone
    Prolactin
    Progesterone
    Estradiol
    Capsules
    Brain
    Median Eminence
    Oils
    Suprachiasmatic Nucleus
    Preoptic Area
    Anterior Hypothalamic Nucleus
    Sesame Oil
    Ovariectomy
    Ether
    Estrogens
    Steroids

    ASJC Scopus subject areas

    • Cell Biology

    Cite this

    Effects of estradiol and progesterone on plasma gonadotropins, prolactin, and LHRH in specific brain areas of ovariectomized rats. / Wise, Phyllis M; Camp-Grossman, P.; Barraclough, C. A.

    In: Biology of reproduction, Vol. 24, No. 4, 01.01.1981, p. 820-830.

    Research output: Contribution to journalArticle

    Wise, Phyllis M ; Camp-Grossman, P. ; Barraclough, C. A. / Effects of estradiol and progesterone on plasma gonadotropins, prolactin, and LHRH in specific brain areas of ovariectomized rats. In: Biology of reproduction. 1981 ; Vol. 24, No. 4. pp. 820-830.
    @article{f7d134bc836c466d900982bf64d1f805,
    title = "Effects of estradiol and progesterone on plasma gonadotropins, prolactin, and LHRH in specific brain areas of ovariectomized rats",
    abstract = "The authors examined the effects of physiological concentrations of estradiol (E2) and progesterone (P) on plasma LH, FSH, and prolactin (PRL) and on LHRH concentrations in microdissected brain regions in ovariectomized (OVX) rats. One week after ovariectomy (Day 0), rats received Silastic capsules containing only sesame oil or 37.5, 75.0 or 150 μg E2/ml of oil s.c. The E2 capsules produced plasma estrogen concentrations of 7.0, 9.6, or 15.4 pg/ml, respectively, on Day 2 whereas oil-treated controls had 5.4 pg/ml of E2 in plasma. All three E2-treated groups of rats had LH surges of comparable magnitude during the afternoon of Day 2. Two Silastic capsules of P (50 mg/ml) were implanted at 0900 h on Day 2 into E2-treated rats. In animals in which E2 levels were 7.0 or 9.6 pg/ml, P only moderately amplified the LH surges. However, when plasma E2 concentrations reached 15 pg/ml, P treatment evoked a massive release of LH and advanced the time of release by 1 h. In contrast, the stress of inserting oil capsules into ether-anesthetized E2-treated rats at 0900 h on Day 2 had no effect on afternoon LH surges in these animals. FSH surges occurred only in rats receiving both E2 and P. PRL surges were induced using the highest concentration of E2 and were advanced in time by P. When the highest dose of E2 was used, LHRH concentrations in the median eminence (ME) increased prior to and decreased during the E2 P-induced surge. These changes were not paralleled by any changes in the other anterior brain regions we examined (suprachiasmatic preoptic nucleus, suprachiasmatic nucleus, medial preoptic nucleus, anterior hypothalamic nucleus, and retrochiasmatic area). No changes in LHRH concentrations were observed under any other steroid treatment regimen. Higher plasma E2 concentrations induced by the 150 μg/ml E2 capsule were correlated with higher LHRH concentrations in the median eminence concomitant with a parallel trend in some of the anterior brain areas.",
    author = "Wise, {Phyllis M} and P. Camp-Grossman and Barraclough, {C. A.}",
    year = "1981",
    month = "1",
    day = "1",
    doi = "10.1095/biolreprod24.4.820",
    language = "English (US)",
    volume = "24",
    pages = "820--830",
    journal = "Biology of Reproduction",
    issn = "0006-3363",
    publisher = "Society for the Study of Reproduction",
    number = "4",

    }

    TY - JOUR

    T1 - Effects of estradiol and progesterone on plasma gonadotropins, prolactin, and LHRH in specific brain areas of ovariectomized rats

    AU - Wise, Phyllis M

    AU - Camp-Grossman, P.

    AU - Barraclough, C. A.

    PY - 1981/1/1

    Y1 - 1981/1/1

    N2 - The authors examined the effects of physiological concentrations of estradiol (E2) and progesterone (P) on plasma LH, FSH, and prolactin (PRL) and on LHRH concentrations in microdissected brain regions in ovariectomized (OVX) rats. One week after ovariectomy (Day 0), rats received Silastic capsules containing only sesame oil or 37.5, 75.0 or 150 μg E2/ml of oil s.c. The E2 capsules produced plasma estrogen concentrations of 7.0, 9.6, or 15.4 pg/ml, respectively, on Day 2 whereas oil-treated controls had 5.4 pg/ml of E2 in plasma. All three E2-treated groups of rats had LH surges of comparable magnitude during the afternoon of Day 2. Two Silastic capsules of P (50 mg/ml) were implanted at 0900 h on Day 2 into E2-treated rats. In animals in which E2 levels were 7.0 or 9.6 pg/ml, P only moderately amplified the LH surges. However, when plasma E2 concentrations reached 15 pg/ml, P treatment evoked a massive release of LH and advanced the time of release by 1 h. In contrast, the stress of inserting oil capsules into ether-anesthetized E2-treated rats at 0900 h on Day 2 had no effect on afternoon LH surges in these animals. FSH surges occurred only in rats receiving both E2 and P. PRL surges were induced using the highest concentration of E2 and were advanced in time by P. When the highest dose of E2 was used, LHRH concentrations in the median eminence (ME) increased prior to and decreased during the E2 P-induced surge. These changes were not paralleled by any changes in the other anterior brain regions we examined (suprachiasmatic preoptic nucleus, suprachiasmatic nucleus, medial preoptic nucleus, anterior hypothalamic nucleus, and retrochiasmatic area). No changes in LHRH concentrations were observed under any other steroid treatment regimen. Higher plasma E2 concentrations induced by the 150 μg/ml E2 capsule were correlated with higher LHRH concentrations in the median eminence concomitant with a parallel trend in some of the anterior brain areas.

    AB - The authors examined the effects of physiological concentrations of estradiol (E2) and progesterone (P) on plasma LH, FSH, and prolactin (PRL) and on LHRH concentrations in microdissected brain regions in ovariectomized (OVX) rats. One week after ovariectomy (Day 0), rats received Silastic capsules containing only sesame oil or 37.5, 75.0 or 150 μg E2/ml of oil s.c. The E2 capsules produced plasma estrogen concentrations of 7.0, 9.6, or 15.4 pg/ml, respectively, on Day 2 whereas oil-treated controls had 5.4 pg/ml of E2 in plasma. All three E2-treated groups of rats had LH surges of comparable magnitude during the afternoon of Day 2. Two Silastic capsules of P (50 mg/ml) were implanted at 0900 h on Day 2 into E2-treated rats. In animals in which E2 levels were 7.0 or 9.6 pg/ml, P only moderately amplified the LH surges. However, when plasma E2 concentrations reached 15 pg/ml, P treatment evoked a massive release of LH and advanced the time of release by 1 h. In contrast, the stress of inserting oil capsules into ether-anesthetized E2-treated rats at 0900 h on Day 2 had no effect on afternoon LH surges in these animals. FSH surges occurred only in rats receiving both E2 and P. PRL surges were induced using the highest concentration of E2 and were advanced in time by P. When the highest dose of E2 was used, LHRH concentrations in the median eminence (ME) increased prior to and decreased during the E2 P-induced surge. These changes were not paralleled by any changes in the other anterior brain regions we examined (suprachiasmatic preoptic nucleus, suprachiasmatic nucleus, medial preoptic nucleus, anterior hypothalamic nucleus, and retrochiasmatic area). No changes in LHRH concentrations were observed under any other steroid treatment regimen. Higher plasma E2 concentrations induced by the 150 μg/ml E2 capsule were correlated with higher LHRH concentrations in the median eminence concomitant with a parallel trend in some of the anterior brain areas.

    UR - http://www.scopus.com/inward/record.url?scp=0019798439&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=0019798439&partnerID=8YFLogxK

    U2 - 10.1095/biolreprod24.4.820

    DO - 10.1095/biolreprod24.4.820

    M3 - Article

    C2 - 7018601

    AN - SCOPUS:0019798439

    VL - 24

    SP - 820

    EP - 830

    JO - Biology of Reproduction

    JF - Biology of Reproduction

    SN - 0006-3363

    IS - 4

    ER -