Effects of endurance exercise training on inflammatory circulating progenitor cell content in lean and obese adults

Grace M. Niemiro, Jacob M. Allen, Lucy J. Mailing, Naiman A Khan, Hannah Diane Holscher, Jeffrey A Woods, Michael De Lisio

Research output: Contribution to journalArticle

Abstract

Key points: Chronic inflammation underlies many of the health decrements associated with obesity. Circulating progenitor cells can sense and respond to inflammatory stimuli, increasing the local inflammatory response within tissues. Here we show that 6 weeks of endurance exercise training significantly decreases inflammatory circulating progenitor cells in obese adults. These findings provide novel cellular mechanisms for the beneficial effects of exercise in obese adults. Abstract: Circulating progenitor cells (CPCs) and subpopulations are normally found in the bone marrow, but can migrate to peripheral tissues to participate in local inflammation and/or remodelling. The purpose of this study was to compare the CPC response, particularly the inflammatory-primed haematopoietic stem and progenitor (HSPC) subpopulation, to a 6 week endurance exercise training (EET) intervention between lean and obese adults. Seventeen healthy weight (age: 23.9 ± 5.4 years, body mass index (BMI): 22.0 ± 2.6 kg m−2) and 10 obese (age: 29.0 ± 8.0 years, BMI: 33.1 ± 6.0 kg m−2) previously sedentary adults participated in an EET. Blood was collected before and after EET for quantification of CPCs and subpopulations via flow cytometry, colony forming unit assays and plasma concentrations of C-X-C motif chemokine 12 (CXCL12), granulocyte-colony stimulating factor (G-CSF), and chemokine (C-C motif) ligand 2 (CCL2). Exercise training reduced the number of circulating HSPCs and adipose tissue-derived mesenchymal stem cells (AT-MSCs). EET increased the colony forming potential of granulocytes and macrophages irrespective of BMI. EET reduced the number of HSPCs expressing the chemokine receptor CCR2 and the pro-inflammatory marker TLR4. EET-induced changes in adipose tissue-derived MSCs and bone marrow-derived MSCs were negatively related to changes in absolute fitness. Our results indicate that EET, regardless of BMI status, decreases CPCs and subpopulations, particularly those primed for contribution to tissue inflammation.

Original languageEnglish (US)
Pages (from-to)2811-2822
Number of pages12
JournalJournal of Physiology
Volume596
Issue number14
DOIs
StatePublished - Jul 15 2018

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Stem Cells
Exercise
Body Mass Index
Inflammation
Adipose Tissue
Bone Marrow
Colony-Forming Units Assay
Chemokine CXCL12
CXC Chemokines
Chemokine CCL2
Chemokine Receptors
Granulocyte Colony-Stimulating Factor
Mesenchymal Stromal Cells
Granulocytes
Flow Cytometry
Obesity
Macrophages
Weights and Measures
Health

Keywords

  • Hematopoietic stem cells
  • Inflammation
  • Mesenchymal stem cell
  • Obesity
  • chemokine c receptor 2

ASJC Scopus subject areas

  • Physiology

Cite this

Effects of endurance exercise training on inflammatory circulating progenitor cell content in lean and obese adults. / Niemiro, Grace M.; Allen, Jacob M.; Mailing, Lucy J.; Khan, Naiman A; Holscher, Hannah Diane; Woods, Jeffrey A; De Lisio, Michael.

In: Journal of Physiology, Vol. 596, No. 14, 15.07.2018, p. 2811-2822.

Research output: Contribution to journalArticle

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