TY - JOUR
T1 - Effects of dietary vegetable oils on mammary lipid-related genes in holstein dairy cows
AU - Vargas-Bello-pérez, Einar
AU - Geldsetzer-Mendoza, Carolina
AU - Cancino-Padilla, Nathaly
AU - Morales, María Sol
AU - Leskinen, Heidi
AU - Garnsworthy, Philip C.
AU - Loor, Juan J.
AU - Romero, Jaime
N1 - Funding: This study was sponsored by a research grant from FONDECYT 1170400 (Fondo Nacional de Desarrollo Científico y Tecnológico, Chile) and Vicerrectoría de Investigación of Pontificia Universidad Católica de Chile (Proyecto Puente P1608).
This study was sponsored by a research grant from FONDECYT 1170400 (Fondo Nacional de Desarrollo Cient?fico y Tecnol?gico, Chile) and Vicerrector?a de Investigaci?n of Pontificia Universidad Cat?lica de Chile (Proyecto Puente P1608). Acknowledgments: We would like to thank Pietro Sciarresi-Arechabala, Carolina Garrido, Daniela Pi?a, Stefanie Vyhmeister, Ricardo Gebauer, Lucas Arze and Jos? Mar?a Godoy for technical assistance and Fundaci?n Agro U.C. for the animal facilities and assistance in obtaining research data. We also thank Comercial e Industrial Soho S.A. for kindly donating the olive oil.
PY - 2020/1
Y1 - 2020/1
N2 - This study analyzed effects of vegetable oils fed to dairy cows on abundance of genes related to lipid metabolism in milk somatic cells (MSC). During 63 days, 15 cows were allocated to 3 treatments: a control diet with no added lipid the same diet supplemented with olive oil (OO, 30 g/kg DM) or hydrogenated vegetable oil (HVO, 30 g/kg DM). On days 21, 42 and 63, MSC were obtained from all cows. Relative abundance of genes involved in lipid metabolism in MSC from cows fed control on days 42 and 63 was compared with relative abundance at day 21 to evaluate fold-changes. Those genes without changes over the time were selected to analyze effects of OO and HVO. Compared with control, on day 42, PLIN2 and THRSP were upregulated by OO. Compared with control, on day 21, HVO up regulated ACACA, down regulated FABP3, and on day 63 THRSP and FABP4 were down regulated. Dietary oil supplementation (3% DM) had a modest nutrigenomic effect on different biological functions such as acetate and FA activation and intra-cellular transport, lipid droplet formation, and transcription regulation in MSC.
AB - This study analyzed effects of vegetable oils fed to dairy cows on abundance of genes related to lipid metabolism in milk somatic cells (MSC). During 63 days, 15 cows were allocated to 3 treatments: a control diet with no added lipid the same diet supplemented with olive oil (OO, 30 g/kg DM) or hydrogenated vegetable oil (HVO, 30 g/kg DM). On days 21, 42 and 63, MSC were obtained from all cows. Relative abundance of genes involved in lipid metabolism in MSC from cows fed control on days 42 and 63 was compared with relative abundance at day 21 to evaluate fold-changes. Those genes without changes over the time were selected to analyze effects of OO and HVO. Compared with control, on day 42, PLIN2 and THRSP were upregulated by OO. Compared with control, on day 21, HVO up regulated ACACA, down regulated FABP3, and on day 63 THRSP and FABP4 were down regulated. Dietary oil supplementation (3% DM) had a modest nutrigenomic effect on different biological functions such as acetate and FA activation and intra-cellular transport, lipid droplet formation, and transcription regulation in MSC.
KW - Gene abundance
KW - Mammary gland
KW - Milk somatic cells
KW - Olive oil
KW - Transcriptomic
UR - https://www.scopus.com/pages/publications/85077385525
UR - https://www.scopus.com/pages/publications/85077385525#tab=citedBy
U2 - 10.3390/ani10010057
DO - 10.3390/ani10010057
M3 - Article
C2 - 31892210
AN - SCOPUS:85077385525
SN - 2076-2615
VL - 10
JO - Animals
JF - Animals
IS - 1
M1 - 57
ER -