Effects of dietary neutral detergent fiber and starch ratio on rumen epithelial cell morphological structure and gene expression in dairy cows

L. Ma, M. Zhao, L. S. Zhao, J. C. Xu, J. J. Loor, D. P. Bu

Research output: Contribution to journalArticle

Abstract

This study was designed to investigate the effect of dietary neutral detergent fiber to starch ratio on rumen epithelial morphological structure and gene expression. Eight primiparous dairy cows including 4 ruminally fistulated cows were assigned to 4 total mixed rations with neutral detergent fiber to starch ratios of 0.86, 1.18, 1.63, and 2.34 in a replicated 4 × 4 Latin square design. The duration of each period was 21 d including 14 d for adaptation and 7 d for sampling. Rumen epithelial papillae were collected from the ruminally fistulated cows for morphological structure examination and mRNA expression analysis using quantitative real-time PCR of several genes related to volatile fatty acid absorption and metabolism, and cellular growth. Increasing dietary neutral detergent fiber to starch ratio resulted in a linear increase in the thickness of the stratum spinosum and basale. In contrast, expression of HMGCS2 (encoding the rate-limiting enzyme in the synthesis of ketone bodies) decreased linearly, whereas the expression of MCT2 (encoding a transporter of volatile fatty acid) increased linearly with increasing dietary neutral detergent fiber to starch ratio. As dietary neutral detergent fiber to starch ratio increased, expression of IGFBP5 (a gene related to the growth of rumen epithelial papillae) decreased, whereas IGFBP6 expression increased. Both of these IGFBP genes are regulated by short-chain fatty acids. Overall, the data indicate that dietary neutral detergent fiber to starch ratio can alter the thickness of the rumen epithelial papillae partly through changes in expression of genes associated with regulating volatile fatty acid absorption, metabolism, and cell growth.

Original languageEnglish (US)
Pages (from-to)3705-3712
Number of pages8
JournalJournal of Dairy Science
Volume100
Issue number5
DOIs
StatePublished - May 2017

Fingerprint

Rumen
Starch
Detergents
neutral detergent fiber
rumen
epithelial cells
dairy cows
Volatile Fatty Acids
Epithelial Cells
starch
Gene Expression
gene expression
volatile fatty acids
Growth
Genes
cows
ketone bodies
Ketone Bodies
Insulin-Like Growth Factor Binding Proteins
insulin-like growth factor binding proteins

Keywords

  • dairy cow
  • gene expression
  • neutral detergent fiber to starch ratio
  • rumen morphological

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Effects of dietary neutral detergent fiber and starch ratio on rumen epithelial cell morphological structure and gene expression in dairy cows. / Ma, L.; Zhao, M.; Zhao, L. S.; Xu, J. C.; Loor, J. J.; Bu, D. P.

In: Journal of Dairy Science, Vol. 100, No. 5, 05.2017, p. 3705-3712.

Research output: Contribution to journalArticle

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