Effects of dietary clays on performance and intestinal mucus barrier of broiler chicks challenged with salmonella enterica serovar typhimurium and on goblet cell function in vitro

J. A.S. Almeida, N. P. Ponnuraj, J. J. Lee, P. Utterback, H Rex Gaskins, Ryan Neil Dilger, J. E. Pettigrew

Research output: Contribution to journalArticle

Abstract

In vivo and in vitro experiments were conducted to test for beneficial effects of dietary clays on broiler chicks challenged with Salmonella enterica serovar Typhimurium and to explore potential mechanisms. First, two hundred forty 1-d-old male broilers (initial BW: 41.6 ± 0.4 g) were allotted in a 2 × 4 factorial arrangement in a randomized complete block design. There were 2 infection treatments (with or without Salmonella) and 4 diets: basal (BAS), 0.3% smectite A (SMA), 0.3% smectite B, and 0.3% zeolite. The Salmonella reduced (P < 0.05) the growth rate of chicks fed the BAS, and feeding clay largely restored it (challenge × diet interaction, P < 0.05). Goblet cell number and size were increased (P < 0.05) by Salmonella in chicks fed the BAS and were reduced (P < 0.05) in Salmonella-challenged chicks by feeding SMA. Villus height was reduced by the Salmonella challenge in the chicks fed dietary clays (P < 0.01) but not in chicks fed the BAS (interaction P < 0.05). A human adenocarcinoma cell line (LS174T) was cultured in vitro in 3 separate experiments in the absence or presence of 3 concentrations (0.05, 0.10, and 0.50%) of SMA. Expression of mucin 2 (MUC2), resistin-like molecule β (RELMβ), and trefoil factor 3 (TFF3) were determined by real-time reverse-transcription PCR. The expression of RELMβ was increased and expression of MUC2 was reduced (P < 0.05) by 0.10% SMA. Also, LS174T cells were cultured without or with SMA (0.05 and 0.10%) and the medium and cell lysate were analyzed for RELMβ using an immunoblot assay. Protein expression of RELMβ in the cell lysate was reduced (P < 0.05) by SMA addition but increased in the medium, indicating that SMA increased secretion of RELMβ, thus depleting the cell and concentrating this protein in the medium. In conclusion, the dietary clays restored the growth depression caused by Salmonella, and changes in goblet cell function may contribute to the benefits of one of the clays, specifically SMA.

Original languageEnglish (US)
Pages (from-to)839-847
Number of pages9
JournalPoultry science
Volume93
Issue number4
DOIs
StatePublished - Apr 2014

Fingerprint

goblet cells
smectite
mucus
Salmonella Typhimurium
resistin
broiler chickens
clay
Salmonella
chicks
mucins
cells
concentrating
villi
adenocarcinoma
diet
protein synthesis
reverse transcriptase polymerase chain reaction
cell lines
secretion
assays

Keywords

  • Chick
  • Clay
  • Goblet cell
  • LS174T cell line
  • Salmonella enterica serovar Typhimurium

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Effects of dietary clays on performance and intestinal mucus barrier of broiler chicks challenged with salmonella enterica serovar typhimurium and on goblet cell function in vitro. / Almeida, J. A.S.; Ponnuraj, N. P.; Lee, J. J.; Utterback, P.; Gaskins, H Rex; Dilger, Ryan Neil; Pettigrew, J. E.

In: Poultry science, Vol. 93, No. 4, 04.2014, p. 839-847.

Research output: Contribution to journalArticle

@article{23e7692e6f87495ca800351fd76dbc76,
title = "Effects of dietary clays on performance and intestinal mucus barrier of broiler chicks challenged with salmonella enterica serovar typhimurium and on goblet cell function in vitro",
abstract = "In vivo and in vitro experiments were conducted to test for beneficial effects of dietary clays on broiler chicks challenged with Salmonella enterica serovar Typhimurium and to explore potential mechanisms. First, two hundred forty 1-d-old male broilers (initial BW: 41.6 ± 0.4 g) were allotted in a 2 × 4 factorial arrangement in a randomized complete block design. There were 2 infection treatments (with or without Salmonella) and 4 diets: basal (BAS), 0.3{\%} smectite A (SMA), 0.3{\%} smectite B, and 0.3{\%} zeolite. The Salmonella reduced (P < 0.05) the growth rate of chicks fed the BAS, and feeding clay largely restored it (challenge × diet interaction, P < 0.05). Goblet cell number and size were increased (P < 0.05) by Salmonella in chicks fed the BAS and were reduced (P < 0.05) in Salmonella-challenged chicks by feeding SMA. Villus height was reduced by the Salmonella challenge in the chicks fed dietary clays (P < 0.01) but not in chicks fed the BAS (interaction P < 0.05). A human adenocarcinoma cell line (LS174T) was cultured in vitro in 3 separate experiments in the absence or presence of 3 concentrations (0.05, 0.10, and 0.50{\%}) of SMA. Expression of mucin 2 (MUC2), resistin-like molecule β (RELMβ), and trefoil factor 3 (TFF3) were determined by real-time reverse-transcription PCR. The expression of RELMβ was increased and expression of MUC2 was reduced (P < 0.05) by 0.10{\%} SMA. Also, LS174T cells were cultured without or with SMA (0.05 and 0.10{\%}) and the medium and cell lysate were analyzed for RELMβ using an immunoblot assay. Protein expression of RELMβ in the cell lysate was reduced (P < 0.05) by SMA addition but increased in the medium, indicating that SMA increased secretion of RELMβ, thus depleting the cell and concentrating this protein in the medium. In conclusion, the dietary clays restored the growth depression caused by Salmonella, and changes in goblet cell function may contribute to the benefits of one of the clays, specifically SMA.",
keywords = "Chick, Clay, Goblet cell, LS174T cell line, Salmonella enterica serovar Typhimurium",
author = "Almeida, {J. A.S.} and Ponnuraj, {N. P.} and Lee, {J. J.} and P. Utterback and Gaskins, {H Rex} and Dilger, {Ryan Neil} and Pettigrew, {J. E.}",
year = "2014",
month = "4",
doi = "10.3382/ps.2013-03587",
language = "English (US)",
volume = "93",
pages = "839--847",
journal = "Poultry Science",
issn = "0032-5791",
publisher = "Poultry Science Association",
number = "4",

}

TY - JOUR

T1 - Effects of dietary clays on performance and intestinal mucus barrier of broiler chicks challenged with salmonella enterica serovar typhimurium and on goblet cell function in vitro

AU - Almeida, J. A.S.

AU - Ponnuraj, N. P.

AU - Lee, J. J.

AU - Utterback, P.

AU - Gaskins, H Rex

AU - Dilger, Ryan Neil

AU - Pettigrew, J. E.

PY - 2014/4

Y1 - 2014/4

N2 - In vivo and in vitro experiments were conducted to test for beneficial effects of dietary clays on broiler chicks challenged with Salmonella enterica serovar Typhimurium and to explore potential mechanisms. First, two hundred forty 1-d-old male broilers (initial BW: 41.6 ± 0.4 g) were allotted in a 2 × 4 factorial arrangement in a randomized complete block design. There were 2 infection treatments (with or without Salmonella) and 4 diets: basal (BAS), 0.3% smectite A (SMA), 0.3% smectite B, and 0.3% zeolite. The Salmonella reduced (P < 0.05) the growth rate of chicks fed the BAS, and feeding clay largely restored it (challenge × diet interaction, P < 0.05). Goblet cell number and size were increased (P < 0.05) by Salmonella in chicks fed the BAS and were reduced (P < 0.05) in Salmonella-challenged chicks by feeding SMA. Villus height was reduced by the Salmonella challenge in the chicks fed dietary clays (P < 0.01) but not in chicks fed the BAS (interaction P < 0.05). A human adenocarcinoma cell line (LS174T) was cultured in vitro in 3 separate experiments in the absence or presence of 3 concentrations (0.05, 0.10, and 0.50%) of SMA. Expression of mucin 2 (MUC2), resistin-like molecule β (RELMβ), and trefoil factor 3 (TFF3) were determined by real-time reverse-transcription PCR. The expression of RELMβ was increased and expression of MUC2 was reduced (P < 0.05) by 0.10% SMA. Also, LS174T cells were cultured without or with SMA (0.05 and 0.10%) and the medium and cell lysate were analyzed for RELMβ using an immunoblot assay. Protein expression of RELMβ in the cell lysate was reduced (P < 0.05) by SMA addition but increased in the medium, indicating that SMA increased secretion of RELMβ, thus depleting the cell and concentrating this protein in the medium. In conclusion, the dietary clays restored the growth depression caused by Salmonella, and changes in goblet cell function may contribute to the benefits of one of the clays, specifically SMA.

AB - In vivo and in vitro experiments were conducted to test for beneficial effects of dietary clays on broiler chicks challenged with Salmonella enterica serovar Typhimurium and to explore potential mechanisms. First, two hundred forty 1-d-old male broilers (initial BW: 41.6 ± 0.4 g) were allotted in a 2 × 4 factorial arrangement in a randomized complete block design. There were 2 infection treatments (with or without Salmonella) and 4 diets: basal (BAS), 0.3% smectite A (SMA), 0.3% smectite B, and 0.3% zeolite. The Salmonella reduced (P < 0.05) the growth rate of chicks fed the BAS, and feeding clay largely restored it (challenge × diet interaction, P < 0.05). Goblet cell number and size were increased (P < 0.05) by Salmonella in chicks fed the BAS and were reduced (P < 0.05) in Salmonella-challenged chicks by feeding SMA. Villus height was reduced by the Salmonella challenge in the chicks fed dietary clays (P < 0.01) but not in chicks fed the BAS (interaction P < 0.05). A human adenocarcinoma cell line (LS174T) was cultured in vitro in 3 separate experiments in the absence or presence of 3 concentrations (0.05, 0.10, and 0.50%) of SMA. Expression of mucin 2 (MUC2), resistin-like molecule β (RELMβ), and trefoil factor 3 (TFF3) were determined by real-time reverse-transcription PCR. The expression of RELMβ was increased and expression of MUC2 was reduced (P < 0.05) by 0.10% SMA. Also, LS174T cells were cultured without or with SMA (0.05 and 0.10%) and the medium and cell lysate were analyzed for RELMβ using an immunoblot assay. Protein expression of RELMβ in the cell lysate was reduced (P < 0.05) by SMA addition but increased in the medium, indicating that SMA increased secretion of RELMβ, thus depleting the cell and concentrating this protein in the medium. In conclusion, the dietary clays restored the growth depression caused by Salmonella, and changes in goblet cell function may contribute to the benefits of one of the clays, specifically SMA.

KW - Chick

KW - Clay

KW - Goblet cell

KW - LS174T cell line

KW - Salmonella enterica serovar Typhimurium

UR - http://www.scopus.com/inward/record.url?scp=84898027809&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84898027809&partnerID=8YFLogxK

U2 - 10.3382/ps.2013-03587

DO - 10.3382/ps.2013-03587

M3 - Article

C2 - 24706960

AN - SCOPUS:84898027809

VL - 93

SP - 839

EP - 847

JO - Poultry Science

JF - Poultry Science

SN - 0032-5791

IS - 4

ER -