Effects of allelochemical isolated from phragmites communis on algal membrane permeability

Efflux of K⁺, Mg²⁺, Ca²⁺ ions from algal cells as signals of cell membrane permeability, inductively coupled plasma mass spectrometry (ICP-MS) as detection method of ions, the present research investigated effects of allelochemical eathyl-2-methyl acetoacetate (EMA) isolated from Phragmites communis on cell membrane permeability of Microcystis aeruginosa, Chlorella pyrenoidosa and Chlorella vulagaris. The results showed that, when the cells were boiled for 10 min and the membrane was destroyed absolutely, the K⁺ efflux of M. aeruginosa and C. pyrenoidosa were 1.45 and 1.59 μg · (10⁹ cell)^-1, respectively. When the concentration of EMA was 2 mg · L^-1, the K⁺ efflux of M. aeruginosa and C. pyrenoidosa were 1.38 and 1.40 μg-(10⁹ cell)^-1, respectively. The K⁺ efflux of M. aeruginosa and C. pyrenoidosa reached 1.44 and 1.58 μg · (10⁽ cell)^-1 while the EMA was 4 mg · L^-1. When the concentrations were 2 mg · L^-1 or 4 mg · L^-1 the K⁺ efflux reached more than 95% of the total ion amount in M. aeruginosa and C. pyrenoidosa cells. But when EMA concentration was 4 mg · L^-1, K⁺ efflux of C. vulagaris was 0.64 μg · (10 cell)^-1, which was only 31.5% of total K⁺ amount in C. vulagaris. Effects EMA on efflux of Mg²⁺ and Ca²⁺ were similar to those of K+. The results indicated that EMA destroyed the cell membrane of M. aeruginosa and C. pyrenoidosa but not C. vulagaris. This is one of the mechanisms of EMA species-selective antialgal.