Effects of allelochemical EMA isolated from phragmites communis on algal cell membrane lipid and ultrastructure

Feng Min Li, Hong Ying Hu, Yun Xiao Chong, Yu Jie Men, Mei Ting Guo

Research output: Contribution to journalArticlepeer-review

Abstract

In order to reveal the antialgal mechanisms of allelochemicals, effects of the allelochemical eathyl-2-methyl acetoacetate (EMA) on cell membrane lipid and ultrastructure of Chlorella pyrenoidosa, Microcystis aeruginosa and Chlorella vulagaris were studied in this paper. The lipid fatty acids of the algal membrane were isolated following the Bligh and Dye method and quantified by gas chromatograph/mass spectrometry. The ultrastructure of algal cells was observed with TEM. The results showed that EMA increased the contents of linolenic acid and linolic acid with increment of 14%, while decreased the content of myristic acid and cetylic acid in C. pyrenoidosa membrane. The content of unsaturated fatty acids C18:1 and C18:2 increased 12% and 10% in M. aeruginosa with the addition of EMA, while the content of saturated fatty acids C18:0 and C16:0 decreased. EMA showed no significant change in the fatty acid composition in C. vulagaris under the experiment condition. EMA broke off cell wall of C. pyrenoidosa and M. aeruginosa. EMA damaged the cell membrane and the inclusion of algal cell leaked out. Nuclear and mitochondrial structure was damaged with the addition of EMA. EMA showed no significant change in the ultrastructure of C. vulagaris.

Original languageEnglish (US)
Pages (from-to)1534-1538
Number of pages5
JournalHuanjing Kexue/Environmental Science
Volume28
Issue number7
StatePublished - Jul 1 2007

Keywords

  • Algal
  • Allelopathy
  • Cell membrane
  • Eathyl-2-methyl acetoacetate
  • Lipid fatty acid
  • Ultrastructure

ASJC Scopus subject areas

  • Environmental Science(all)

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