Effects of 1-isothiocyanato-3-(methylsulfinyl)-propane on xenobiotic metabolizing enzymes in rats

Research output: Contribution to journalArticlepeer-review


The glucosinolate hydrolysis product 1-isothiocyanato-3-(methylsulfinyl)-propane (IMSP), also known as iberin, is consumed in the average human (US) diet at approximately 1 μmol/kg/day. The chemoprotective effects observed with the consumption of cruciferous vegetables may be due to the presence of specific glucosinolate hydrolysis products either within the crucifers, or formed after ingestion of the crucifers. The mechanism of chemoprotection may be through selective induction of components of Phase II xenobiotic metabolizing enzymes. The influence of repeated administration of low concentrations of IMSP by gavage on components of Phase I and Phase II xenobiotic metabolizing systems was examined in the liver and small intestine of male Fischer 344 rats. Doses of 1, 10 and 100 μol IMSP/kg, administered by gavage for 7 days, did not alter weight gain, or hepatic and renal weights, relative to body weight, and did not cause any histological lesions. Intestinal glutathione S-transferase (GST) activity and NAD(P)H:quinone reductase (QR) activities were significantly elevated to 3.1 and 8.1 times control values, respectively, at the 100 μmol/kg dose only. The administration of IMSP at 1, 10 or 100 μmol/kg had no significant effect on hepatic Phase I enzyme activities (cytochrome P-450 concentrations, ethoxycoumarin O-deethylase [ECD] and aminopyrine N-demethylase [AND] activities) or Phase II enzyme activities (GST, QR and UDP-glucuronosyltransferase [UDP-GT] activities towards 1-naphthol or 4-hydroxybiphenyl), at any of the doses tested and no effect on intestinal enzyme activities at doses below 100 μmol IMSP/kg. It is concluded that IMSP does not have a significant influence on induction of the Phase I or Phase II xenobiotic metabolizing enzymes in rats when tested at doses approximating those found in the human diet.

Original languageEnglish (US)
Pages (from-to)723-729
Number of pages7
JournalFood and Chemical Toxicology
Issue number10
StatePublished - Oct 1993


  • 1-naphthol GT = microsomal UDP-GT activity towards 1-naphthol
  • 4-HBP = 4-hydroxybiphenyl
  • 4-HBP GT = microsomal UDP-GT activity towards 4-hydroxybiphenyl
  • 4-dinitrobenzene
  • 6-dichlorophenolindophenol
  • AND = aminopyrine N-demethylase
  • ANOVA = analysis of variance
  • CDNB = 1-chloro-2
  • CYP = cytochrome P-450 protein
  • DPIP = 2
  • ECD = ethoxycoumarin O-deethylase
  • GST = glutathione S-transferase
  • IMSB = 1-isothiocyanato-4-(methylsulfiny)-butane
  • IMSP = 1-isothiocyanato-3-(methylsulfinyl)-propane (CAS No. 505-44-2)
  • MSBN = 3-(methylsulfinyl)-butanenitrile (CAS No. 61121-65-1)
  • QR = NAD(P)H:quinone reductase
  • UDP-GT = uridine diphosphate glucurono-syltransferase
  • cyt P450 = cytochrome P-450

ASJC Scopus subject areas

  • Food Science
  • Toxicology


Dive into the research topics of 'Effects of 1-isothiocyanato-3-(methylsulfinyl)-propane on xenobiotic metabolizing enzymes in rats'. Together they form a unique fingerprint.

Cite this