Effect of tissue staining in quantitative phase imaging

Sungbea Ban, Eunjung Min, Yujin Ahn, Gabriel Popescu, Woonggyu Jung

Research output: Contribution to journalArticlepeer-review


Quantitative phase imaging (QPI) is an emerging modality, which enables the identification of abnormalities in tissue based on optical properties. QPI can be applied to any biological specimen due to its label-free imaging capability, but its use in stained tissue is unclear. Here, we study the variability of QPI with the staining dye. Several tissues such as brain, heart and lung were stained with hematoxylin and eosin, and their optical properties compared at 550 and 730 nm. Our results showed that phase and scattering coefficients varied when QPI was used at the absorption wavelength of the staining dye. We also found that the variation of optical properties was dependent on tissue morphology.

Original languageEnglish (US)
Article numbere201700402
JournalJournal of Biophotonics
Issue number8
StatePublished - Aug 2018


  • interference microscopy
  • optical properties
  • phase measurement
  • quantitative phase imaging
  • scattering measurement

ASJC Scopus subject areas

  • General Chemistry
  • General Materials Science
  • General Biochemistry, Genetics and Molecular Biology
  • General Engineering
  • General Physics and Astronomy


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