Effect of tissue staining in quantitative phase imaging

Sungbea Ban, Eunjung Min, Yujin Ahn, Gabriel Popescu, Woonggyu Jung

Research output: Contribution to journalLetter

Abstract

Quantitative phase imaging (QPI) is an emerging modality, which enables the identification of abnormalities in tissue based on optical properties. QPI can be applied to any biological specimen due to its label-free imaging capability, but its use in stained tissue is unclear. Here, we study the variability of QPI with the staining dye. Several tissues such as brain, heart and lung were stained with hematoxylin and eosin, and their optical properties compared at 550 and 730 nm. Our results showed that phase and scattering coefficients varied when QPI was used at the absorption wavelength of the staining dye. We also found that the variation of optical properties was dependent on tissue morphology.

Original languageEnglish (US)
Article numbere201700402
JournalJournal of Biophotonics
Volume11
Issue number8
DOIs
StatePublished - Aug 1 2018

Fingerprint

staining
Tissue
Staining and Labeling
Imaging techniques
optical properties
Optical properties
Coloring Agents
dyes
Dyes
abnormalities
scattering coefficients
Hematoxylin
Eosine Yellowish-(YS)
lungs
brain
emerging
Labels
Brain
Lung
Scattering

Keywords

  • interference microscopy
  • optical properties
  • phase measurement
  • quantitative phase imaging
  • scattering measurement

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

Effect of tissue staining in quantitative phase imaging. / Ban, Sungbea; Min, Eunjung; Ahn, Yujin; Popescu, Gabriel; Jung, Woonggyu.

In: Journal of Biophotonics, Vol. 11, No. 8, e201700402, 01.08.2018.

Research output: Contribution to journalLetter

Ban, Sungbea ; Min, Eunjung ; Ahn, Yujin ; Popescu, Gabriel ; Jung, Woonggyu. / Effect of tissue staining in quantitative phase imaging. In: Journal of Biophotonics. 2018 ; Vol. 11, No. 8.
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