TY - JOUR
T1 - Effect of tissue staining in quantitative phase imaging
AU - Ban, Sungbea
AU - Min, Eunjung
AU - Ahn, Yujin
AU - Popescu, Gabriel
AU - Jung, Woonggyu
N1 - Funding Information:
2018 Research Fund of UNIST, Grant/Award Number: 1.180042.01; National Research Foundation of Korea, Grant/Award Number: 2017009566; National Science Foundation
Funding Information:
This work was partly supported by National Research Foundation of Korea (NRF) grant of the Korea government (MSIP) (No. 2017009566) and 2018 Research Fund (1.180042.01) of Ulsan National Institute of Science and Technology (UNIST). This work was supported in part by the National Science Foundation (CBET-0939511 STC, DBI 1450962 EAGER, IIP-1353368, and CBET-1040461 MRI)
Publisher Copyright:
© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2018/8
Y1 - 2018/8
N2 - Quantitative phase imaging (QPI) is an emerging modality, which enables the identification of abnormalities in tissue based on optical properties. QPI can be applied to any biological specimen due to its label-free imaging capability, but its use in stained tissue is unclear. Here, we study the variability of QPI with the staining dye. Several tissues such as brain, heart and lung were stained with hematoxylin and eosin, and their optical properties compared at 550 and 730 nm. Our results showed that phase and scattering coefficients varied when QPI was used at the absorption wavelength of the staining dye. We also found that the variation of optical properties was dependent on tissue morphology.
AB - Quantitative phase imaging (QPI) is an emerging modality, which enables the identification of abnormalities in tissue based on optical properties. QPI can be applied to any biological specimen due to its label-free imaging capability, but its use in stained tissue is unclear. Here, we study the variability of QPI with the staining dye. Several tissues such as brain, heart and lung were stained with hematoxylin and eosin, and their optical properties compared at 550 and 730 nm. Our results showed that phase and scattering coefficients varied when QPI was used at the absorption wavelength of the staining dye. We also found that the variation of optical properties was dependent on tissue morphology.
KW - interference microscopy
KW - optical properties
KW - phase measurement
KW - quantitative phase imaging
KW - scattering measurement
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U2 - 10.1002/jbio.201700402
DO - 10.1002/jbio.201700402
M3 - Article
C2 - 29726110
AN - SCOPUS:85047785059
SN - 1864-063X
VL - 11
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 8
M1 - e201700402
ER -