Effect of temperature and water availability during late maturation of the soybean seed on germ and cotyledon isoflavone content and composition

BACKGROUND: Isoflavone content in soybean seeds is strongly influenced by both environment and genotype. However, little is known about the effect of environment and genotype on isoflavones in germ versus cotyledons. To determine the effect of temperature and soil moisture status during soybean seed development on seed isoflavone concentration and composition, a set of two French and three US cultivars of similar maturity were grown in the greenhouse. At the R6 growth stage, plants were subjected to one of three night/day temperature regimes (13/23°, 18/28° or 23/33°C) in either optimal or sub-optimal soil water conditions. RESULTS: In cotyledons, a three- to six-fold variation in total isoflavone content was observed between the high and low temperature treatments, whereas the germ contents had less than a two-fold variation. Soil water supply had less effect than temperature on the isoflavone contents and compositions. In both seed parts, the isoflavone concentrations were highly dependent on the cultivar. CONCLUSION: These results show that isoflavone content and composition in cotyledon and germ are unrelated and it should be possible to independently manipulate these seed traits through plant breeding and crop management systems.