Effect of retinoic acid upon mevalonic acid 2 14C incorporation into lipids in an isolated rat liver fraction

J. W. Erdman, J. G. Elliott, P. A. Lachance

Research output: Contribution to journalArticle

Abstract

Methodology was developed to study the effects of 400 μM retinoic acid (OIC) upon the timed incorporation of 14C from mevalonic acid-2-14C into various lipids in the post-mitochondrial supernatant fraction of rat liver. Liver fractions were incubated with various cofactors and mevalonic acid, with and without OIC, for time periods up to 120 minutes. The reaction was stopped, lipids separated with thin layer chromatography, the activities in bands were recorded, and Rf values compared to various lipid standards. The results showed that OIC depressed 14C incorporation into cholesterol and cholesterol esters while OIC increased 14C accumulation into bands corresponding to fatty acids, mono-, di-, and triglycerides. OIC caused increased activity in squalene and in two other possible intermediates of cholesterol biosynthesis after 10 or 20 minutes incubation, suggesting that OIC initially inhibits cholesterol biosynthesis causing the build-up of intermediates. These results demonstrate that 400 μM OIC has a direct effect upon in vitro lipid synthesis in an isolated rat liver system.

Original languageEnglish (US)
Pages (from-to)37-46
Number of pages10
JournalNutrition Reports International
Volume16
Issue number1
StatePublished - Jan 1 1977
Externally publishedYes

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science
  • Biochemistry
  • Endocrinology

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