Effect of resistant starch on hydrolysis and fermentation of corn starch for ethanol

Vivek Sharma, Kent D. Rausch, James V. Graeber, Shelly J. Schmidt, Philip Buriak, M. E. Tumbleson, Vijay Singh

Research output: Contribution to journalArticlepeer-review


Starch samples with 0% or 30% amylose were subjected to four different liquefaction enzyme treatments (at various temperature and pH conditions) followed by simultaneous saccharification and fermentation (SSF). Resistant starch (RS) measurements were conducted for the initial starch sample, after liquefaction and after SSF. Initial RS was higher for 30% amylose starch samples (16.53 g/100 g sample) compared with 0% amylose (0.76 g/100 g sample). Higher initial RS resulted in lower conversion of starch into sugars and lower final ethanol yields. The four enzymes hydrolyzed RS, but in varying amounts. Higher temperature liquefaction hydrolyzed a larger portion of RS, resulting in higher ethanol concentrations and lower final residual solids (non-fermentables), whereas lower temperature liquefaction hydrolyzed a smaller portion of RS and resulted in lower ethanol concentrations and higher final residual solids. Decreases in resistant starch after high temperature liquefaction were 55% to 74%, whereas low temperature liquefaction decreases were 11% to 43%. For all enzyme treatments, RS content of starch samples decreased further after SSF.

Original languageEnglish (US)
Pages (from-to)800-811
Number of pages12
JournalApplied Biochemistry and Biotechnology
Issue number3
StatePublished - Feb 2010


  • Amylose
  • Dry grind corn process
  • Enzymes
  • Ethanol
  • Fermentation
  • Liquefaction
  • Resistant starch

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology


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