Interleukin-12 (IL-12) is a pro-inflammatory cytokine known for its role in immunity, and previous studies have shown that IL-12 provides mitigation of radiation injury. In this study, we utilize a multimodal microscopy system equipped with second harmonic generation (SHG) and fluorescence lifetime imaging microscopy (FLIM) to examine the effect of IL-12 on collagen structure and cellular metabolic activity in vivo during skin wound healing. This preliminary study illustrates the highly dynamic and heterogeneous in vivo microenvironment of the wounded skin. In addition, results suggest that IL-12 triggers a significantly more rapid and greater cellular metabolic response in the wounded animals. These results can elucidate insights into the response mechanism of IL-12 in both wound healing and acute radiation syndrome.

Original languageEnglish (US)
Article number243477
Pages (from-to)4277-4287
Number of pages11
JournalBiomedical Optics Express
Issue number11
StatePublished - Oct 6 2015


  • Diagnostic applications of nonlinear optics
  • Fluorescence microscopy
  • Medical and biological imaging
  • Nonlinear microscopy
  • Time-resolved imaging

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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