Effect of reaction mode on biohydrogen production and its microbial diversity

Buchun Si, Zhidan Liu, Yuanhui Zhang, Jiaming Li, Xin Hui Xing, Baoming Li, Na Duan, Haifeng Lu

Research output: Contribution to journalArticlepeer-review


Biohydrogen production using the high-rate reactor is promising due to its ability of maintaining higher biomass concentrations through forming granules or biofilms. This study investigated the effect of reaction mode on hydrogen fermentation by comparing an upflow anaerobic sludge blanket (UASB) and a packed bed reactor (PBR). UASB and PBR were operated for 120 days at hydraulic retention time (HRT) of 24-12 h and organic loading rates (OLRs) of 0.96-15.36 g COD/L/d. Both UASB and PBR achieved maximal hydrogen production rates as 2.77 ± 0.18 and 1.28 ± 0.12 L/L/d, respectively, at 15.36 g COD/L/d, corresponding to hydrogen yields of 1.44 ± 0.01 and 0.67 ± 0.06 mol/mol glucose. Illumina MiSeq sequencing results revealed Clostridium sp. was the dominant microbial consortium for hydrogen production in UASB (92.1%) and PBR (71.7%). Compared to UASB, PBR showed a greater microbial diversity of ethanol and lactic acid producers, and may be more favorable for methanogenesis and homoacetogenesis. This study demonstrates that reaction mode significantly influenced microbial diversity and biohydrogen production.

Original languageEnglish (US)
Pages (from-to)3191-3200
Number of pages10
JournalInternational Journal of Hydrogen Energy
Issue number8
StatePublished - Mar 2 2015


  • Biohydrogen production
  • Microbial diversity
  • Packed bed reactor
  • Reaction mode
  • Upflow anaerobic sludge blanket

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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