Effect of Preparation Method on the Protein Profile of Equine Amnion Dressings

Annette M. McCoy, Justine Arrington, Peter M. Yau

Research output: Contribution to journalArticlepeer-review

Abstract

The protein content of amnion is thought to be the primary contributor to its efficacy as a biological dressing for wounds. Protein elution into antibiotic processing media has been reported, but the effect of antiseptic-based processing methods is unknown. Amniotic membranes were collected from eight healthy mares. Samples were collected after removal of gross debris. Tissues were subsequently divided and processed with either 0.05% chlorhexidine or 2% iodine/0.25% acetic acid. After protein extraction and trypsin digestion, the proteins were labeled with 8-plex iTRAQ tags, combined, and analyzed by high-resolution liquid chromatography-mass spectrometry. The MaxQuant-Perseus software suite was used to identify and quantify sample proteins, with functional annotation performed in PANTHER. There were 220 unique proteins identified, of which 144 were found in all individuals and across all conditions, several with a known role in wound healing. Contrary to expectations, processing did not significantly alter the protein content of the amnion tissue. Limitations include the small sample size and single time point. These results suggest that either processing method is acceptable for use in the preparation of equine amnion dressings. The role of expressed proteins in the biological activity of amnion dressings remains to be elucidated.

Original languageEnglish (US)
Pages (from-to)2676-2685
Number of pages10
JournalJournal of Proteome Research
Volume18
Issue number6
DOIs
StatePublished - Jun 7 2019

Keywords

  • horse
  • isobaric tags
  • tissue proteomics
  • wound management

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

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