Effect of pH control on biohythane production and microbial structure in an innovative multistage anaerobic hythane reactor (MAHR)

Hao Yang, Buchun Si, Sijie Huang, Zhidan Liu, Yuanhui Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

An innovative multistage anaerobic hythane reactor (MAHR) which combines an internal biofilm (MH) and an external up-flow sludge blanket (MM) was proposed to produce biohythane from wastewater. The effect of pH on its biohythane production and microbial diversity was performed. Results showed that the maximum hydrogen production rate (4.900 L/L/d) was achieved at a pH of 6.0, in comparison to a maximum methane production rate of 10.271 L/L/d at a pH of 6.5. In addition, a suitable hythane (H2/(H2+CH4) of 16.06%) production can be achieved in MH after the initial pH was adjusted from 7.0 to 6.5, and a relatively high methane yield (271.34 mL CH4/gCOD) was obtained in MM. Illumina Miseq sequencing results revealed that decreasing pH led to an increase of the acidogenesis families (Eubacteriaceae, Ruminococcaceae) in MH and an increase of hydrogenotrophic methanogens (Methanobacteriaceae) in MM. The Methanosaetaceae gradually occupied a major portion after a long period of recovery. This work demonstrated the unique advantages of MAHR for the biohythane production under optimal pH conditions.

Original languageEnglish (US)
Pages (from-to)4193-4204
Number of pages12
JournalInternational Journal of Hydrogen Energy
Volume45
Issue number7
DOIs
StatePublished - Feb 7 2020

Keywords

  • Anaerobic digestion
  • Biogas production
  • Biohythane production
  • Influent pH control
  • Microbial structure
  • Multistage anaerobic hythane reactor

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

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