Effect of microcolony formation on the adherence of pseudomonas aeruginosa to human buccal epithelial cells

P. Doig, Richard I Tapping, P. Mankinen-Irvin, R. T. Irvin

Research output: Contribution to journalArticle

Abstract

Pseudomonas aeruginosa is thought to initiate respiratory infections in susceptible individuals by adhering to and colonising the respiratory epithelium. We have examined the binding properties of P. aeruginosa strain P1 to human buccal epithelial cell (BEC)s and found that it may bind as a single bacterium or as an alginate encased microcolony. Examination of the binding of P1 to BECs revealed that single bacteria bound to BECs via a single class of adhesin-receptor site interactions, where the apparent association constant of binding (Ka) was 4.6 ± 10-9 ml/CFU and the maximum number of bacteria that could bind per BEC (N) was 223 bacteria/BEC. Cultures that produced micro-colonies bound to BECs via two classes of adhesin-receptor site interactions. The first was identical to the class employed by single bacteria and was due to the presence of single bacteria in the culture and did not represent microcolony binding to the BECs. The second class of adhesin-receptor site interactions had a Ka of 4.7 ± 10-10ml/ CFU and N of 3388 bacteria/BEC. This second class of interactions was due to the binding of microcolonies to BECs. These data indicated that single bacteria bound to the BEC with a higher affinity than microcolonies. While the adherence of microcolonies to BECs was not as efficient as single cells, substantially higher numbers of bacteria could attach to an epithelial cell.

Original languageEnglish (US)
Pages (from-to)203-209
Number of pages7
JournalMicrobial Ecology in Health and Disease
Volume2
Issue number3
DOIs
StatePublished - Jan 1 1989

Fingerprint

Cheek
Pseudomonas aeruginosa
Epithelial Cells
Bacteria
Respiratory Mucosa
Respiratory Tract Infections

Keywords

  • Bacterial binding
  • Bacterial colonisation
  • Microcolony
  • Pseudomonas aeruginosa

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Effect of microcolony formation on the adherence of pseudomonas aeruginosa to human buccal epithelial cells. / Doig, P.; Tapping, Richard I; Mankinen-Irvin, P.; Irvin, R. T.

In: Microbial Ecology in Health and Disease, Vol. 2, No. 3, 01.01.1989, p. 203-209.

Research output: Contribution to journalArticle

Doig, P. ; Tapping, Richard I ; Mankinen-Irvin, P. ; Irvin, R. T. / Effect of microcolony formation on the adherence of pseudomonas aeruginosa to human buccal epithelial cells. In: Microbial Ecology in Health and Disease. 1989 ; Vol. 2, No. 3. pp. 203-209.
@article{0efce2137281441aa0fe96227091c1dc,
title = "Effect of microcolony formation on the adherence of pseudomonas aeruginosa to human buccal epithelial cells",
abstract = "Pseudomonas aeruginosa is thought to initiate respiratory infections in susceptible individuals by adhering to and colonising the respiratory epithelium. We have examined the binding properties of P. aeruginosa strain P1 to human buccal epithelial cell (BEC)s and found that it may bind as a single bacterium or as an alginate encased microcolony. Examination of the binding of P1 to BECs revealed that single bacteria bound to BECs via a single class of adhesin-receptor site interactions, where the apparent association constant of binding (Ka) was 4.6 ± 10-9 ml/CFU and the maximum number of bacteria that could bind per BEC (N) was 223 bacteria/BEC. Cultures that produced micro-colonies bound to BECs via two classes of adhesin-receptor site interactions. The first was identical to the class employed by single bacteria and was due to the presence of single bacteria in the culture and did not represent microcolony binding to the BECs. The second class of adhesin-receptor site interactions had a Ka of 4.7 ± 10-10ml/ CFU and N of 3388 bacteria/BEC. This second class of interactions was due to the binding of microcolonies to BECs. These data indicated that single bacteria bound to the BEC with a higher affinity than microcolonies. While the adherence of microcolonies to BECs was not as efficient as single cells, substantially higher numbers of bacteria could attach to an epithelial cell.",
keywords = "Bacterial binding, Bacterial colonisation, Microcolony, Pseudomonas aeruginosa",
author = "P. Doig and Tapping, {Richard I} and P. Mankinen-Irvin and Irvin, {R. T.}",
year = "1989",
month = "1",
day = "1",
doi = "10.3109/08910608909140219",
language = "English (US)",
volume = "2",
pages = "203--209",
journal = "Microbial Ecology in Health and Disease",
issn = "0891-060X",
publisher = "Co-Action Publishing",
number = "3",

}

TY - JOUR

T1 - Effect of microcolony formation on the adherence of pseudomonas aeruginosa to human buccal epithelial cells

AU - Doig, P.

AU - Tapping, Richard I

AU - Mankinen-Irvin, P.

AU - Irvin, R. T.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - Pseudomonas aeruginosa is thought to initiate respiratory infections in susceptible individuals by adhering to and colonising the respiratory epithelium. We have examined the binding properties of P. aeruginosa strain P1 to human buccal epithelial cell (BEC)s and found that it may bind as a single bacterium or as an alginate encased microcolony. Examination of the binding of P1 to BECs revealed that single bacteria bound to BECs via a single class of adhesin-receptor site interactions, where the apparent association constant of binding (Ka) was 4.6 ± 10-9 ml/CFU and the maximum number of bacteria that could bind per BEC (N) was 223 bacteria/BEC. Cultures that produced micro-colonies bound to BECs via two classes of adhesin-receptor site interactions. The first was identical to the class employed by single bacteria and was due to the presence of single bacteria in the culture and did not represent microcolony binding to the BECs. The second class of adhesin-receptor site interactions had a Ka of 4.7 ± 10-10ml/ CFU and N of 3388 bacteria/BEC. This second class of interactions was due to the binding of microcolonies to BECs. These data indicated that single bacteria bound to the BEC with a higher affinity than microcolonies. While the adherence of microcolonies to BECs was not as efficient as single cells, substantially higher numbers of bacteria could attach to an epithelial cell.

AB - Pseudomonas aeruginosa is thought to initiate respiratory infections in susceptible individuals by adhering to and colonising the respiratory epithelium. We have examined the binding properties of P. aeruginosa strain P1 to human buccal epithelial cell (BEC)s and found that it may bind as a single bacterium or as an alginate encased microcolony. Examination of the binding of P1 to BECs revealed that single bacteria bound to BECs via a single class of adhesin-receptor site interactions, where the apparent association constant of binding (Ka) was 4.6 ± 10-9 ml/CFU and the maximum number of bacteria that could bind per BEC (N) was 223 bacteria/BEC. Cultures that produced micro-colonies bound to BECs via two classes of adhesin-receptor site interactions. The first was identical to the class employed by single bacteria and was due to the presence of single bacteria in the culture and did not represent microcolony binding to the BECs. The second class of adhesin-receptor site interactions had a Ka of 4.7 ± 10-10ml/ CFU and N of 3388 bacteria/BEC. This second class of interactions was due to the binding of microcolonies to BECs. These data indicated that single bacteria bound to the BEC with a higher affinity than microcolonies. While the adherence of microcolonies to BECs was not as efficient as single cells, substantially higher numbers of bacteria could attach to an epithelial cell.

KW - Bacterial binding

KW - Bacterial colonisation

KW - Microcolony

KW - Pseudomonas aeruginosa

UR - http://www.scopus.com/inward/record.url?scp=0542382412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0542382412&partnerID=8YFLogxK

U2 - 10.3109/08910608909140219

DO - 10.3109/08910608909140219

M3 - Article

VL - 2

SP - 203

EP - 209

JO - Microbial Ecology in Health and Disease

JF - Microbial Ecology in Health and Disease

SN - 0891-060X

IS - 3

ER -