Effect of lycopene on lipid peroxidation and glutathione-dependent enzymes induced by T-2 toxin in vivo

Myriam Leal, Armando Shimada, Felipe Ruíz, Elvira González De Mejía

Research output: Contribution to journalArticlepeer-review

Abstract

Lycopene, obtained from fresh tomatoes, was incorporated into the chicks diet. The treatments were: (1) Control, (2) 1.5 mg T-2 toxin/kg body weight/day; (3) 25 mg lycopene/kg body weight/day, (4) 1.5 mg T-2 toxin plus 25 mg lycopene/kg body weight/day. Male broiler chicks, 7-28 days of age, were provided with feed and water ad libitum. Every 7 days, malondialdehyde (MDA) and glutathione (GSH) levels, and enzymatic activities of glutathione-S-transferase (GST), γ-glutamyltransferase (GGT) and glutathione peroxidase (GP) were evaluated in liver homogenates. Compared to the controls after 7 days of treatment, T-2 toxin increased hepatic MDA concentration (128%). A significant consumption of endogenous antioxidant GSH (45%) was induced as well as a marked increase in hepatic enzymatic activities of GST, GGT, and GP (312, 187, and 324%, respectively). Addition of T-2 plus lycopene, at an approximate ratio of 1:17 in the diet, diminished some parameters measured (P<0.05). Apparently lycopene participated as an antioxidant agent and also protecting the cellular level of GSH.

Original languageEnglish (US)
Pages (from-to)1-10
Number of pages10
JournalToxicology Letters
Volume109
Issue number1-2
DOIs
StatePublished - Sep 20 1999
Externally publishedYes

Keywords

  • Glutathione
  • Lycopene
  • T-2 toxin

ASJC Scopus subject areas

  • Toxicology

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