TY - JOUR
T1 - Effect of lycopene on lipid peroxidation and glutathione-dependent enzymes induced by T-2 toxin in vivo
AU - Leal, Myriam
AU - Shimada, Armando
AU - Ruíz, Felipe
AU - González De Mejía, Elvira
N1 - Funding Information:
This study was supported by the Miguel Hidalgo Research Program (SIGHO-CONACyT) Querétaro, México. We gratefully acknowledge the supply of animals and foodstuffs by Pilgrim’s Pride S.A. de C.V. (Querétaro, México). The authors thank Q.B. Ma. Eugenia Valtierra for her excellent technical assistance.
PY - 1999/9/20
Y1 - 1999/9/20
N2 - Lycopene, obtained from fresh tomatoes, was incorporated into the chicks diet. The treatments were: (1) Control, (2) 1.5 mg T-2 toxin/kg body weight/day; (3) 25 mg lycopene/kg body weight/day, (4) 1.5 mg T-2 toxin plus 25 mg lycopene/kg body weight/day. Male broiler chicks, 7-28 days of age, were provided with feed and water ad libitum. Every 7 days, malondialdehyde (MDA) and glutathione (GSH) levels, and enzymatic activities of glutathione-S-transferase (GST), γ-glutamyltransferase (GGT) and glutathione peroxidase (GP) were evaluated in liver homogenates. Compared to the controls after 7 days of treatment, T-2 toxin increased hepatic MDA concentration (128%). A significant consumption of endogenous antioxidant GSH (45%) was induced as well as a marked increase in hepatic enzymatic activities of GST, GGT, and GP (312, 187, and 324%, respectively). Addition of T-2 plus lycopene, at an approximate ratio of 1:17 in the diet, diminished some parameters measured (P<0.05). Apparently lycopene participated as an antioxidant agent and also protecting the cellular level of GSH.
AB - Lycopene, obtained from fresh tomatoes, was incorporated into the chicks diet. The treatments were: (1) Control, (2) 1.5 mg T-2 toxin/kg body weight/day; (3) 25 mg lycopene/kg body weight/day, (4) 1.5 mg T-2 toxin plus 25 mg lycopene/kg body weight/day. Male broiler chicks, 7-28 days of age, were provided with feed and water ad libitum. Every 7 days, malondialdehyde (MDA) and glutathione (GSH) levels, and enzymatic activities of glutathione-S-transferase (GST), γ-glutamyltransferase (GGT) and glutathione peroxidase (GP) were evaluated in liver homogenates. Compared to the controls after 7 days of treatment, T-2 toxin increased hepatic MDA concentration (128%). A significant consumption of endogenous antioxidant GSH (45%) was induced as well as a marked increase in hepatic enzymatic activities of GST, GGT, and GP (312, 187, and 324%, respectively). Addition of T-2 plus lycopene, at an approximate ratio of 1:17 in the diet, diminished some parameters measured (P<0.05). Apparently lycopene participated as an antioxidant agent and also protecting the cellular level of GSH.
KW - Glutathione
KW - Lycopene
KW - T-2 toxin
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U2 - 10.1016/S0378-4274(99)00062-4
DO - 10.1016/S0378-4274(99)00062-4
M3 - Article
C2 - 10514025
AN - SCOPUS:0032886044
SN - 0378-4274
VL - 109
SP - 1
EP - 10
JO - Toxicology Letters
JF - Toxicology Letters
IS - 1-2
ER -