Effect of ice fraction and dilution factor on the antifreeze activity in the hemolymph of the cerambycid beetle Rhagium inquisitor

Karl Erik Zachariassen; Arthur L. DeVries; Ben Hunt; Erlend Kristiansen

doi:10.1016/S0011-2240(02)00014-7

Effect of ice fraction and dilution factor on the antifreeze activity in the hemolymph of the cerambycid beetle Rhagium inquisitor

Karl Erik Zachariassen, Arthur L. DeVries, Ben Hunt, Erlend Kristiansen

Evolution, Ecology, and Behavior

Research output: Contribution to journal › Article › peer-review

Abstract

The freezing-melting hysteresis in a given volume of hemolymph from the cerambycid beetle Rhagium inquisitor was linearly and negatively related to the logarithm of the mass fraction of ice in the sample. When the ice fraction dropped by a factor of 10, the hysteresis activity increased by about 2°C. When the hemolymph was diluted, the hysteresis activity was linearly and negatively related to the logarithm of the dilution factor. Dilution of the hemolymph by a factor of 2 led to a 1°C reduction in hysteresis activity. In the diluted samples, the ice growth took place along the a-axes, implying that the antifreeze peptides of insects block ice growth along the c-axis, in addition to the a-axis.

Original language	English (US)
Pages (from-to)	132-141
Number of pages	10
Journal	Cryobiology
Volume	44
Issue number	2
DOIs	https://doi.org/10.1016/S0011-2240(02)00014-7
State	Published - Apr 2002

Keywords

Antifreeze proteins
Cerambycidae
Insects
Thermal hysteresis

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Agricultural and Biological Sciences

Online availability

10.1016/S0011-2240(02)00014-7

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title = "Effect of ice fraction and dilution factor on the antifreeze activity in the hemolymph of the cerambycid beetle Rhagium inquisitor",

abstract = "The freezing-melting hysteresis in a given volume of hemolymph from the cerambycid beetle Rhagium inquisitor was linearly and negatively related to the logarithm of the mass fraction of ice in the sample. When the ice fraction dropped by a factor of 10, the hysteresis activity increased by about 2°C. When the hemolymph was diluted, the hysteresis activity was linearly and negatively related to the logarithm of the dilution factor. Dilution of the hemolymph by a factor of 2 led to a 1°C reduction in hysteresis activity. In the diluted samples, the ice growth took place along the a-axes, implying that the antifreeze peptides of insects block ice growth along the c-axis, in addition to the a-axis.",

keywords = "Antifreeze proteins, Cerambycidae, Insects, Thermal hysteresis",

author = "Zachariassen, {Karl Erik} and DeVries, {Arthur L.} and Ben Hunt and Erlend Kristiansen",

note = "Funding Information: This work was funded by Norwegian University of Science and Technology and the University of Illinois at Urbana-Champaign. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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doi = "10.1016/S0011-2240(02)00014-7",

language = "English (US)",

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AU - Zachariassen, Karl Erik

AU - DeVries, Arthur L.

AU - Hunt, Ben

AU - Kristiansen, Erlend

N1 - Funding Information: This work was funded by Norwegian University of Science and Technology and the University of Illinois at Urbana-Champaign. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2002/4

Y1 - 2002/4

N2 - The freezing-melting hysteresis in a given volume of hemolymph from the cerambycid beetle Rhagium inquisitor was linearly and negatively related to the logarithm of the mass fraction of ice in the sample. When the ice fraction dropped by a factor of 10, the hysteresis activity increased by about 2°C. When the hemolymph was diluted, the hysteresis activity was linearly and negatively related to the logarithm of the dilution factor. Dilution of the hemolymph by a factor of 2 led to a 1°C reduction in hysteresis activity. In the diluted samples, the ice growth took place along the a-axes, implying that the antifreeze peptides of insects block ice growth along the c-axis, in addition to the a-axis.

AB - The freezing-melting hysteresis in a given volume of hemolymph from the cerambycid beetle Rhagium inquisitor was linearly and negatively related to the logarithm of the mass fraction of ice in the sample. When the ice fraction dropped by a factor of 10, the hysteresis activity increased by about 2°C. When the hemolymph was diluted, the hysteresis activity was linearly and negatively related to the logarithm of the dilution factor. Dilution of the hemolymph by a factor of 2 led to a 1°C reduction in hysteresis activity. In the diluted samples, the ice growth took place along the a-axes, implying that the antifreeze peptides of insects block ice growth along the c-axis, in addition to the a-axis.

KW - Antifreeze proteins

KW - Cerambycidae

KW - Insects

KW - Thermal hysteresis

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DO - 10.1016/S0011-2240(02)00014-7

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SN - 0011-2240

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EP - 141

JO - Cryobiology

JF - Cryobiology

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ER -

Effect of ice fraction and dilution factor on the antifreeze activity in the hemolymph of the cerambycid beetle Rhagium inquisitor

Abstract

Keywords

ASJC Scopus subject areas

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