Effect of DNA length and H4 acetylation on the thermal stability of reconstituted nucleosome particles

Joseph S. Siino, Peter M. Yau, Brian S. Imai, Joe M. Gatewood, E. Morton Bradbury

Research output: Contribution to journalArticle

Abstract

To examine the factors involved with nucleosome stability, we reconstituted nonacetylated particles containing various lengths (192, 162, and 152base pairs) of DNA onto the Lytechinus variegatus nucleosome positioning sequence in the absence of linker histone. We characterized the particles and examined their thermal stability. DNA of less than chromatosome length (168 base pairs) produces particles with altered denaturation profiles, possibly caused by histone rearrangement in those core-like particles. We also examined the effects of tetra-acetylation of histone H4 on the thermal stability of reconstituted nucleosome particles. Tetra-acetylation of H4 reduces the nucleosome thermal stability by 0.8°C as compared with nonacetylated particles. This difference is close to values published comparing bulk nonacetylated nucleosomes and core particles to ones enriched for core histone acetylation, suggesting that H4 acetylation has a dominant effect on nucleosome particle energetics.

Original languageEnglish (US)
Pages (from-to)885-891
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume302
Issue number4
DOIs
StatePublished - Mar 21 2003

Keywords

  • Acetylation
  • Nucleosome
  • Thermal denaturation

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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