TY - JOUR
T1 - Effect of different exogenous fatty acids on the cytosolic triacylglycerol content in bovine mammary cells
AU - Vargas-Bello-Pérez, Einar
AU - Loor, Juan J.
AU - Garnsworthy, Philip C.
N1 - Funding Information:
Einar Vargas-Bello-Pérez was supported by a PhD studentship (196739) from Consejo Nacional de Ciencia y Tecnología (CONACYT- México). We thank Linda Sheldrick, Dongfang Li, and Zoë Daniel for technical assistance. Statistical advice was provided by Jim Craigon.
Funding Information:
Einar Vargas-Bello-Pérez was supported by a PhD studentship ( 196739 ) from Consejo Nacional de Ciencia y Tecnología (CONACYT- México) . We thank Linda Sheldrick, Dongfang Li, and Zoë Daniel for technical assistance. Statistical advice was provided by Jim Craigon.
Publisher Copyright:
© 2019 Chinese Association of Animal Science and Veterinary Medicine
PY - 2019/6
Y1 - 2019/6
N2 - The objective of this study was to determine how cytosolic triacylglycerols (TAG) are stored in mammary cells and whether this depends on the individual chemical configuration of fatty acids (FA). This objective was accomplished by addition of different FA to a FA-free medium used to culture mammary alveolar cells-large T antigen cells (MAC-T). Treatments consisted of adding FA (palmitate, stearate, oleate, linoleate, rumenic acid [CLA], elaidate and vaccinate) solutions to the medium at 100, 200, 300 and 400 mmol/L concentrations for a 24-h incubation period. At the end of each incubation period, cytosolic TAG, DNA and protein contents were measured. Palmitate, vaccenate, linoleate and CLA increased (P < 0.05) cytosolic TAG (μg/mg protein). Palmitate and CLA increased (P < 0.05) cytosolic TAG adjusted for DNA content. Overall, effects on cytosolic TAG accumulation depended on individual FA structure (chain length, degree of saturation, and number and orientation of FA double bonds). In addition, the long-chain FA used in this study did not have a detrimental effect on MAC-T cells as indicated by cytosolic protein and DNA contents reflecting their biological role in lipid accumulation.
AB - The objective of this study was to determine how cytosolic triacylglycerols (TAG) are stored in mammary cells and whether this depends on the individual chemical configuration of fatty acids (FA). This objective was accomplished by addition of different FA to a FA-free medium used to culture mammary alveolar cells-large T antigen cells (MAC-T). Treatments consisted of adding FA (palmitate, stearate, oleate, linoleate, rumenic acid [CLA], elaidate and vaccinate) solutions to the medium at 100, 200, 300 and 400 mmol/L concentrations for a 24-h incubation period. At the end of each incubation period, cytosolic TAG, DNA and protein contents were measured. Palmitate, vaccenate, linoleate and CLA increased (P < 0.05) cytosolic TAG (μg/mg protein). Palmitate and CLA increased (P < 0.05) cytosolic TAG adjusted for DNA content. Overall, effects on cytosolic TAG accumulation depended on individual FA structure (chain length, degree of saturation, and number and orientation of FA double bonds). In addition, the long-chain FA used in this study did not have a detrimental effect on MAC-T cells as indicated by cytosolic protein and DNA contents reflecting their biological role in lipid accumulation.
KW - Long chain fatty acid
KW - Mammary cells
KW - Trans fatty acid
KW - Triacylglycerol accumulation
KW - Vaccenic acid
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U2 - 10.1016/j.aninu.2018.09.002
DO - 10.1016/j.aninu.2018.09.002
M3 - Article
C2 - 31193913
AN - SCOPUS:85059509159
SN - 2405-6545
VL - 5
SP - 202
EP - 208
JO - Animal Nutrition
JF - Animal Nutrition
IS - 2
ER -