Effect of carotenoids against genotoxicity of diethylnitrosamine on rat hepatocytes

T. García-Gasca, S. Fatell, S. Villa-Trevino, E. De González Mejía

Research output: Contribution to journalArticlepeer-review

Abstract

Carotenoids have been considered as special nutrients due to their biological activity as provitamin A compounds, and because of their natural antioxidant and anticarcinogenic properties. The main objective of this study was to evaluate the protective effect of carotenoids against the genotoxic cellular damage induced by diethylnitrosamine (DEN), a potent hepatocarcinogen. Normal and freshly isolated hepatocytes were cultured as the biological system. Concentrations of 2.5 and 5 μM DEN caused 1.3 and 2.0 times more DNA T3H incorporation, respectively, when compared with control cells. Pure carotenoids, β-carotene (50 μM), lutein (1 μM) and a carotenoid extract from green peppers (1 μM eq. lutein) were used as functional nutrients to protect the cells. All the carotenoids studied prevented the genotoxic damage caused by 2.5 μM DEN. When 5 μM DEN was used, only β-carotene and the pepper extract inhibited the damage up to 30- 40%. Carotenoids provide a dose-dependent protective effect against DNA damage induced by DEN in isolated hepatocytes.

Original languageEnglish (US)
Pages (from-to)691-698
Number of pages8
JournalToxicology in Vitro
Volume12
Issue number6
DOIs
StatePublished - Dec 1998
Externally publishedYes

Keywords

  • Carotenoids
  • Chemoprevention
  • Genotoxicity
  • Nitrosamines

ASJC Scopus subject areas

  • Toxicology

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