Effect of antifreeze glycoproteins on organoid survival during and after hypothermic storage

Guizela Huelsz-Prince, Arthur L. Devries, Huib J. Bakker, Jeroen S.Van Zon, Konrad Meister

Research output: Contribution to journalArticlepeer-review


We study the effect of antifreeze glycoproteins (AFGPs) on the survival of organoids under hypothermic conditions. We find that the survival of organoids in cold conditions depends on their developmental stage. Mature organoids die within 24 h when being stored at 4°C, while cystic organoids can survive up to 48 h. We find that in the presence of AFGPs, the organoid survival is prolonged up to 72 h, irrespective of their developmental stage. Fluorescence microscopy experiments reveal that the AFGPs predominately localize at the cell surface and cover the cell membranes. Our findings support a mechanism in which the positive effect of AFGPs on cell survival during hypothermic storage involves the direct interaction of AFGPs with the cell membrane. Our research highlights organoids as an attractive multicellular model system for studying the action of AFGPs that bridges the gap between single-cell and whole-organ studies.

Original languageEnglish (US)
Article number110
Issue number3
StatePublished - Mar 2019


  • 2019
  • 21
  • Antifreeze glycoproteins
  • Fluorescence microscopy
  • Hypothermic storage
  • March
  • Organoids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology


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