Effect of acetylation (O-factor 5) on the polyclonal antibody response to Salmonella typhimurium O-antigen

Myung L. Kim, James M. Slauch

Research output: Contribution to journalArticle

Abstract

Antibodies directed against lipopolysaccharide (LPS) O-antigen are often critical in the immune response to Gram-negative pathogens. Mice were orally immunized with isogenic strains of Salmonella typhimurium that differ only in a minor modification of O-antigen, namely acetylation, mediated by the oafA locus. To specifically examine the effect of acetylation on the antibody response to O-antigen, antibody titers were determined against both acetylated and unacetylated LPS by ELISA. In mice immunized with an oafA+ strain, the median titer against acetylated LPS was 32-fold higher than the titer against unacetylated LPS. Mice immunized with the oafA- strain had an 8-fold higher titer against unacetylated LPS. Thus, acetylation of O-antigen alters recognition by the vast majority of individual antibodies. This differential antibody recognition of O-antigen had a statistically significant correlation with protection against subsequent challenge with virulent S. typhimurium.

Original languageEnglish (US)
Pages (from-to)83-92
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Volume26
Issue number1
DOIs
StatePublished - Oct 1 1999

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O Antigens
Salmonella typhimurium
Acetylation
Antibody Formation
Lipopolysaccharides
Antibodies
Enzyme-Linked Immunosorbent Assay

Keywords

  • Lipopolysaccharide
  • O-Antigen
  • Polyclonal antibody
  • Salmonella

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Effect of acetylation (O-factor 5) on the polyclonal antibody response to Salmonella typhimurium O-antigen. / Kim, Myung L.; Slauch, James M.

In: FEMS Immunology and Medical Microbiology, Vol. 26, No. 1, 01.10.1999, p. 83-92.

Research output: Contribution to journalArticle

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