Ectopic histone H3S10 phosphorylation causes chromatin structure remodeling in Drosophila

Huai Deng, Xiaomin Bao, Weili Cai, Melissa J. Blacketer, Andrew S. Belmont, Jack Girton, Jørgen Johansen, Kristen M. Johansen

Research output: Contribution to journalArticle

Abstract

Histones are subject to numerous post-translational modifications that correlate with the state of higher-order chromatin structure and gene expression. However, it is not clear whether changes in these epigenetic marks are causative regulatory factors in chromatin structure changes or whether they play a mainly reinforcing or maintenance role. In Drosophila phosphorylation of histone H3S10 in euchromatic chromatin regions by the JIL-1 tandem kinase has been implicated in counteracting heterochromatization and gene silencing. Here we show, using a Lac1-tethering system, that JIL-1 mediated ectopic histone H3S10 phosphorylation is sufficient to induce a change in higher-order chromatin structure from a condensed heterochromatin-like state to a more open euchromatic state. This effect was absent when a 'kinase dead' Lacl-JIL-1 construct without histone H3S10 phosphorylation activity was expressed. Instead, the 'kinase dead' construct had a dominant-negative effect, leading to a disruption of chromatin structure that was associated with a global repression of histone H3S10 phosphorylation levels. These findings provide direct evidence that the epigenetic histone tail modification of H3S10 phosphorylation at interphase can function as a causative regulator of higher-order chromatin structure in Drosophila in vivo.

Original languageEnglish (US)
Pages (from-to)699-705
Number of pages7
JournalDevelopment
Volume135
Issue number4
DOIs
StatePublished - Feb 1 2008

Fingerprint

Chromatin Assembly and Disassembly
Histones
Drosophila
Chromatin
Phosphorylation
cause
gene expression
Phosphotransferases
Epigenomics
gene
Histone Code
repression
Heterochromatin
Interphase
Gene Silencing
Post Translational Protein Processing
effect
Tail
Maintenance
Gene Expression

Keywords

  • Chromatin structure remodeling
  • Drosophila
  • Histone H3S10 phosphorylation
  • JIL-1 kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Deng, H., Bao, X., Cai, W., Blacketer, M. J., Belmont, A. S., Girton, J., ... Johansen, K. M. (2008). Ectopic histone H3S10 phosphorylation causes chromatin structure remodeling in Drosophila. Development, 135(4), 699-705. https://doi.org/10.1242/dev.015362

Ectopic histone H3S10 phosphorylation causes chromatin structure remodeling in Drosophila. / Deng, Huai; Bao, Xiaomin; Cai, Weili; Blacketer, Melissa J.; Belmont, Andrew S.; Girton, Jack; Johansen, Jørgen; Johansen, Kristen M.

In: Development, Vol. 135, No. 4, 01.02.2008, p. 699-705.

Research output: Contribution to journalArticle

Deng, H, Bao, X, Cai, W, Blacketer, MJ, Belmont, AS, Girton, J, Johansen, J & Johansen, KM 2008, 'Ectopic histone H3S10 phosphorylation causes chromatin structure remodeling in Drosophila', Development, vol. 135, no. 4, pp. 699-705. https://doi.org/10.1242/dev.015362
Deng, Huai ; Bao, Xiaomin ; Cai, Weili ; Blacketer, Melissa J. ; Belmont, Andrew S. ; Girton, Jack ; Johansen, Jørgen ; Johansen, Kristen M. / Ectopic histone H3S10 phosphorylation causes chromatin structure remodeling in Drosophila. In: Development. 2008 ; Vol. 135, No. 4. pp. 699-705.
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