Early inductive interactions are involved in restricting cell fates of mesomeres in sea urchin embryos

Jonathan J. Henry, Shonan Amemiya, Gregory A. Wray, Rudolf A. Raff

Research output: Contribution to journalArticle

Abstract

Isolated intact caps of animal blastomeres, obtained from either 8- or 16-cell embryos, differentiate as swollen ectodermal vesicles. These findings agree with earlier studies demonstrating that mesomeres contribute only to larval ectoderm during normal development. In contrast, we find that pairs of mesomeres isolated from 16-cell embryos can differentiate endodermal and mesenchymal cells in a substantial number of cases (23%). Thus, mesomeres have a greater developmental potential than is realized during normal development. Further results support hypotheses that graded distributions of morphogenetic determinants exist within these embryos, since the extent of differentiation of isolated mesomeres is related to the relative position of the third cleavage plane along the animal-vegetal axis. When the third cleavage plane is subequatorial and the resulting animal blastomeres inherit a fraction of the vegetal hemisphere, more cases (39%) differentiate endodermal and mesenchymal cell types. A significant number of mesomere pairs (9-14%), however, can still differentiate endodermal and mesenchymal cells when the mesomeres are formed within the animal hemisphere. Thus, putative vegetal morphogenetic determinants may extend into the animal hemisphere in some cases. Further results indicate a temporal restriction in the developmental potential of mesomeres or mesomere progenitor cells since their differentiative capability is greater if they are isolated earlier during development. Aggregates of isolated mesomere pairs also display a decreased developmental potential when compared to isolated mesomere pairs. These results suggest that associations with adjacent cells (vegetal cells as well as adjacent mesomeres) restrict the development of mesomeres between third and sixth cleavages.

Original languageEnglish (US)
Pages (from-to)140-153
Number of pages14
JournalDevelopmental Biology
Volume136
Issue number1
DOIs
StatePublished - Nov 1989

Fingerprint

Sea Urchins
Embryonic Structures
Blastomeres
Ectoderm
Stem Cells

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Early inductive interactions are involved in restricting cell fates of mesomeres in sea urchin embryos. / Henry, Jonathan J.; Amemiya, Shonan; Wray, Gregory A.; Raff, Rudolf A.

In: Developmental Biology, Vol. 136, No. 1, 11.1989, p. 140-153.

Research output: Contribution to journalArticle

Henry, Jonathan J. ; Amemiya, Shonan ; Wray, Gregory A. ; Raff, Rudolf A. / Early inductive interactions are involved in restricting cell fates of mesomeres in sea urchin embryos. In: Developmental Biology. 1989 ; Vol. 136, No. 1. pp. 140-153.
@article{255413a20a7040268d70780b20117898,
title = "Early inductive interactions are involved in restricting cell fates of mesomeres in sea urchin embryos",
abstract = "Isolated intact caps of animal blastomeres, obtained from either 8- or 16-cell embryos, differentiate as swollen ectodermal vesicles. These findings agree with earlier studies demonstrating that mesomeres contribute only to larval ectoderm during normal development. In contrast, we find that pairs of mesomeres isolated from 16-cell embryos can differentiate endodermal and mesenchymal cells in a substantial number of cases (23{\%}). Thus, mesomeres have a greater developmental potential than is realized during normal development. Further results support hypotheses that graded distributions of morphogenetic determinants exist within these embryos, since the extent of differentiation of isolated mesomeres is related to the relative position of the third cleavage plane along the animal-vegetal axis. When the third cleavage plane is subequatorial and the resulting animal blastomeres inherit a fraction of the vegetal hemisphere, more cases (39{\%}) differentiate endodermal and mesenchymal cell types. A significant number of mesomere pairs (9-14{\%}), however, can still differentiate endodermal and mesenchymal cells when the mesomeres are formed within the animal hemisphere. Thus, putative vegetal morphogenetic determinants may extend into the animal hemisphere in some cases. Further results indicate a temporal restriction in the developmental potential of mesomeres or mesomere progenitor cells since their differentiative capability is greater if they are isolated earlier during development. Aggregates of isolated mesomere pairs also display a decreased developmental potential when compared to isolated mesomere pairs. These results suggest that associations with adjacent cells (vegetal cells as well as adjacent mesomeres) restrict the development of mesomeres between third and sixth cleavages.",
author = "Henry, {Jonathan J.} and Shonan Amemiya and Wray, {Gregory A.} and Raff, {Rudolf A.}",
year = "1989",
month = "11",
doi = "10.1016/0012-1606(89)90137-1",
language = "English (US)",
volume = "136",
pages = "140--153",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Early inductive interactions are involved in restricting cell fates of mesomeres in sea urchin embryos

AU - Henry, Jonathan J.

AU - Amemiya, Shonan

AU - Wray, Gregory A.

AU - Raff, Rudolf A.

PY - 1989/11

Y1 - 1989/11

N2 - Isolated intact caps of animal blastomeres, obtained from either 8- or 16-cell embryos, differentiate as swollen ectodermal vesicles. These findings agree with earlier studies demonstrating that mesomeres contribute only to larval ectoderm during normal development. In contrast, we find that pairs of mesomeres isolated from 16-cell embryos can differentiate endodermal and mesenchymal cells in a substantial number of cases (23%). Thus, mesomeres have a greater developmental potential than is realized during normal development. Further results support hypotheses that graded distributions of morphogenetic determinants exist within these embryos, since the extent of differentiation of isolated mesomeres is related to the relative position of the third cleavage plane along the animal-vegetal axis. When the third cleavage plane is subequatorial and the resulting animal blastomeres inherit a fraction of the vegetal hemisphere, more cases (39%) differentiate endodermal and mesenchymal cell types. A significant number of mesomere pairs (9-14%), however, can still differentiate endodermal and mesenchymal cells when the mesomeres are formed within the animal hemisphere. Thus, putative vegetal morphogenetic determinants may extend into the animal hemisphere in some cases. Further results indicate a temporal restriction in the developmental potential of mesomeres or mesomere progenitor cells since their differentiative capability is greater if they are isolated earlier during development. Aggregates of isolated mesomere pairs also display a decreased developmental potential when compared to isolated mesomere pairs. These results suggest that associations with adjacent cells (vegetal cells as well as adjacent mesomeres) restrict the development of mesomeres between third and sixth cleavages.

AB - Isolated intact caps of animal blastomeres, obtained from either 8- or 16-cell embryos, differentiate as swollen ectodermal vesicles. These findings agree with earlier studies demonstrating that mesomeres contribute only to larval ectoderm during normal development. In contrast, we find that pairs of mesomeres isolated from 16-cell embryos can differentiate endodermal and mesenchymal cells in a substantial number of cases (23%). Thus, mesomeres have a greater developmental potential than is realized during normal development. Further results support hypotheses that graded distributions of morphogenetic determinants exist within these embryos, since the extent of differentiation of isolated mesomeres is related to the relative position of the third cleavage plane along the animal-vegetal axis. When the third cleavage plane is subequatorial and the resulting animal blastomeres inherit a fraction of the vegetal hemisphere, more cases (39%) differentiate endodermal and mesenchymal cell types. A significant number of mesomere pairs (9-14%), however, can still differentiate endodermal and mesenchymal cells when the mesomeres are formed within the animal hemisphere. Thus, putative vegetal morphogenetic determinants may extend into the animal hemisphere in some cases. Further results indicate a temporal restriction in the developmental potential of mesomeres or mesomere progenitor cells since their differentiative capability is greater if they are isolated earlier during development. Aggregates of isolated mesomere pairs also display a decreased developmental potential when compared to isolated mesomere pairs. These results suggest that associations with adjacent cells (vegetal cells as well as adjacent mesomeres) restrict the development of mesomeres between third and sixth cleavages.

UR - http://www.scopus.com/inward/record.url?scp=0024470391&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024470391&partnerID=8YFLogxK

U2 - 10.1016/0012-1606(89)90137-1

DO - 10.1016/0012-1606(89)90137-1

M3 - Article

C2 - 2806717

AN - SCOPUS:0024470391

VL - 136

SP - 140

EP - 153

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -